DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity ofRead more
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Catalog Number
Quantity
18012021
100 Units
18012039
500 Units
Catalog number 18012021
Price (USD)/ 100 units
241.00
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Quantity:
100 Units
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities.
Applications: Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.
Source: Purified from E. coli expressing the Klenow fragment on a plasmid.
Performance and Quality Testing: SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionDNA Polymerase
PolymeraseDNA Polymerase I
Exonuclease Activity3' to 5'
Quantity100 Units
Shipping ConditionApproved for shipment on Wet or Dry Ice
Hot StartNo
Unit Size100 units
Contents & Storage
DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C.