Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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18038018
100 Units
18038042
500 Units
18038067
3 x 500 Units
18038240
5000 Units
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Catalog number 18038018
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88,25
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100 Units
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Price (EUR)
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Taq DNA Polymerase, native, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This native enzyme is purified from Thermus aquaticus YT1 and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.
Features
A choice of recombinant or native enzyme
Amplification of PCR products up to 5 kb in size
Enzyme is licensed and qualified for PCR
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates
RT-PCR
Sequencing ssDNA
Cycle sequencing
Notes
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration5 U/μL
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatStand-alone enzyme
Hot StartNo
No. of Reactions100 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypeDNA Polymerase
Quantity100 Units
Reaction FormatSeparate Components
Shipping ConditionWet or Dry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
PCR MethodqPCR, Standard PCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Taq DNA Polymerase (5 U/μL), 20 μL • 10X PCR buffer (without magnesium), 1.25 mL • 50 mM MgCl2, 1 mL
Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.