MAX Efficiency™ DH5α Competent Cells
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MAX Efficiency™ DH5α Competent Cells
Invitrogen™

MAX Efficiency™ DH5α Competent Cells

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared byRead more
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Catalog number 18258012
Price (USD)/ Each
249.65
Online exclusive
268.00 
Save 18.35 (7%)
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Quantity:
5 x 200 μL
Price (USD)/ Each
249.65
Online exclusive
268.00 
Save 18.35 (7%)
Add to cart
MAX Efficiency™ DH5α Competent Cells
Catalog number18258012
Price (USD)/ Each
249.65
Online exclusive
268.00 
Save 18.35 (7%)
-
Add to cart

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Benefits of MAX Efficiency DH5α Competent Cells:
• Versatile strain for everyday cloning applications
• High quality plasmid preparations
• Useful for generating plasmid libraries 

DH5α competent cells for high transformation efficiency
The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ (or similar) is provided to allow plaque formation.

Features of MAX Efficiency DH5α Competent Cells include:
• Transformation efficiency of >1 x 109 transformants/μg plasmid DNA
• High plasmid yield from the DH5α (endA1) E. coli strain 
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)

Genotype:
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1 

Find the strain and format that fits you needs
DH5α strain and its derivatives available in with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats 
ElectroMAX™ DH5α-E Competent Cells are engineered for electroporation to achieve high transformation efficiency of >1 x 1010
• DH5α T1R competent cells are available in MultiShot format for high throughput applications

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeChemically Competent Cells
Contains F' EpisomeNo
Improves Plasmid QualityYes (endA1)
Cloning Methylated DNANo
Transformation Efficiency LevelHigh Efficiency (>1 x 109 cfu/μg)
Antibiotic Resistance BacterialNo
Cloning Unstable DNANot suitable for cloning unstable DNA
Blue/White ScreeningYes (lacZΔM15)
High-throughput CompatibilityLow
Preparing Unmethylated DNANo
Reduces RecombinationYes (recA1)
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)No
SpeciesE. coli (K12)
FormatTube
Product LineMAX Efficiency™
Quantity5 x 200 μL
Unit SizeEach
Contents & Storage
• Max Efficiency DH5α Competent Cells (5 x 200 μL)
Store Competent Cells at –80°C.

• pUC19 DNA (100 μL at 0.01 μg/mL)
Store pUC19 DNA at –20°C.

• S.O.C. Medium (2 x 6 mL)
Store S.O.C. Medium at 4°C or room temperature.