NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco™ NCTC-109 medium is also suitable for generating and maintaining hybridoma cells.
This NCTC-109 is modified as follows:With | Without |
• Phenol Red | • L-glutamine |
The complete
formulation is available.
Gibco™ NCTC-109 medium is rich in nucleosides, vitamins, coenzymes, reducing agents and other unique ingredients.
Product Intended UseFor
in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
cGMP Manufacturing and Quality SystemGibco™ NCTC-109 medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ NCTC-109 product made in our Scotland facility (21340-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
NCTC-109 contains no proteins, lipids, or growth factors. NCTC-109 medium typically requires supplementation of a growth factor cocktail (for serum free growth) or
Fetal Bovine Serum (FBS). NCTC-109 medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO
2 environment to maintain physiological pH.