1x1 image pixel for data collection
Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
Thermo Scientific™

Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that formRead more
Have Questions?
Catalog NumberQuantity
2165050 mg
Catalog number 21650
Price (USD)
618.00
Each
In stock
Add to cart
Quantity:
50 mg
Recurring order eligible. Learn more »
Request bulk or custom format
Price (USD)
618.00
Each
Add to cart
Ask our AI about this Product
Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Features of Sulfo-LC-SPDP:

Reactive groups: NHS ester and pyridyldithiol
Reactive towards: amino and sulfhydryl groups
• Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
• Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
• Cleavable crosslinker allows separation of crosslinked products
• Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
• Compare to other varieties of SPDP-type reagents, including pegylated forms

Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Applications:
• Reversible crosslinking of proteins
• Adding sulfhydryl groups to proteins by attachment to lysine residues
• Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
• Attaching haptens to carrier proteins for antibody production
• Preparing immunotoxin conjugates

Specifications:
We manufacture Sulfo-LC-SPDP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-LC-SPDP is tested to meet the following minimum specifications.

• Identity: IR scan shows only peaks characteristic of the structure and functional groups of Sulfo-LC-SPDP crosslinker
• Solubility: ≥ 95 mg/mL in DI water
• Purity: ≥ 90% by quantitative NMR (the highest standard for crosslinker purity)

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
SPDP (succinimidyl 3-(2-pyridyldithio)propionate)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityNo
DescriptionSulfo-LC-SPDP
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)527.57
PEGylatedNo
Product LinePierce™
Quantity50 mg
Reactive MoietyPyridyldisulfide, Sulfo-NHS Ester
Shipping ConditionAmbient
SolubilityWater
Spacer Arm Length15.7 Å
Water SolubleYes
Chemical ReactivityAmine-Sulfhydryl
CleavableBy Thiols
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerMedium (10 to 30 Å)
Unit SizeEach
Contents & Storage
Upon receipt store at -20°C protected from moisture.
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

Can you provide the shelf-life for Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)?

Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Figures

Customers who viewed this item also viewed



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
AC404301Certificate of AnalysisApr 02, 202521650
ZI393048Certificate of AnalysisFeb 12, 202521650, DNU-21650
YI376744Certificate of AnalysisSep 26, 202321650
YB355448Certificate of AnalysisAug 10, 202321650
YE360039Certificate of AnalysisJun 03, 202321650
5 results displayed, search above for a specific certificate

Safety Data Sheets

Citations & References (3)

Citations & References
Abstract
Vascular endothelial growth factor selectively targets boronated dendrimers to tumor vasculature.
Authors:Backer MV, Gaynutdinov TI, Patel V, Bandyopadhyaya AK, Thirumamagal BT, Tjarks W, Barth RF, Claffey K, Backer JM
Journal:Mol Cancer Ther
PubMed ID:16170035
'Tumor neovasculature is a potential but, until very recently, unexplored target for boron neutron capture therapy (BNCT) of cancer. In the present report, we describe the construction of a vascular endothelial growth factor (VEGF)-containing bioconjugate that potentially could be used to target up-regulated VEGF receptors (VEGFR), which are overexpressed on ... More
Identification of CKAP4/p63 as a major substrate of the palmitoyl acyltransferase DHHC2, a putative tumor suppressor, using a novel proteomics method.
Authors:Zhang J, Planey SL, Ceballos C, Stevens SM, Keay SK, Zacharias DA
Journal:Mol Cell Proteomics
PubMed ID:18296695
'Protein palmitoylation is the post-translational addition of the 16-carbon fatty acid palmitate to specific cysteine residues by a labile thioester linkage. Palmitoylation is mediated by a family of at least 23 palmitoyl acyltransferases (PATs) characterized by an Asp-His-His-Cys (DHHC) motif. Many palmitoylated proteins have been identified, but PAT-substrate relationships are ... More
Carbon nanotubes enhance CpG uptake and potentiate antiglioma immunity.
Authors:Zhao D, Alizadeh D, Zhang L, Liu W, Farrukh O, Manuel E, Diamond DJ, Badie B,
Journal:Clin Cancer Res
PubMed ID:21088258
Stimulation of toll-like receptor-9 (TLR9) by CpG oligodeoxynucleotides (CpG) has been shown to counteract the immunosuppressive microenvironment and to inhibit tumor growth in glioma models. Because TLR9 is located intracellularly, we hypothesized that methods that enhance its internalization may also potentiate its immunostimulatory response. The goal of this study was ... More
3 total citations

Other products to consider



Share catalog number, name or link