Ham's F-12 Nutrient Mix
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Gibco™

Ham's F-12 Nutrient Mix

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has sinceRead more
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Catalog NumberQuantity
21765029
also known as 21765-029
500 mL
21765037
also known as 21765-037
10 x 500 mL
Catalog number 21765029
also known as 21765-029
Price (EUR)
23,05
Each
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Quantity:
500 mL
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Price (EUR)
23,05
Each
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Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

With: L-glutamine, Phenol Red

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Specifications
Cell TypeMammalian Cells
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeHam's F-12 Nutrient Mix
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesGlutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
Without AdditivesNo HEPES
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Osmolality280 - 310 mOsm/kg
pH7.1 to 7.5
Culture EnvironmentCO2
Culture TypeMammalian Cell Culture
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

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