GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)
GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)
Thermo Scientific™

GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)

Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of aRead more
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Catalog NumberQuantity
2230950 mg
Catalog number 22309
Price (USD)
214.00
Each
In stock
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Quantity:
50 mg
Recurring order eligible. Learn more »
Price (USD)
214.00
Each
Add to cart
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Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).

Features of GMBS:

Reactive groups: NHS ester and maleimide
Reactive toward: amino and sulfhydryl groups
• C4-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-GMBS
• Short aliphatic spacer has low potential for eliciting an immune response

Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
DescriptionGMBS
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)280.23
PEGylatedNo
Product LinePierce™
Quantity50 mg
Reactive MoietyMaleimide, NHS Ester
Shipping ConditionAmbient
SolubilityDMF, DMSO
Spacer Arm Length7.3 Å
Water SolubleNo
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store desiccated at 4°C.
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Frequently asked questions (FAQs)

Can you provide the shelf-life for GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)?

GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
AB407058Certificate of AnalysisFeb 28, 202522309
ZK407016Certificate of AnalysisDec 16, 202422309
ZJ372162Certificate of AnalysisNov 05, 202422309
YK380264Certificate of AnalysisDec 07, 202322309
YF375605Certificate of AnalysisJul 21, 202322309
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Safety Data Sheets

Citations & References (7)

Citations & References
Abstract
Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.
Authors:Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E
Journal:Nucleic Acids Res
PubMed ID:11024189
Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More
Covalent attachment of synthetic DNA to self-assembled monolayer films.
Authors:Chrisey LA, Lee GU, O'Ferrall CE
Journal:Nucleic Acids Res
PubMed ID:8760890
'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More
Recognition of galactan components of pectin by galectin-3.
Authors:Gunning AP, Bongaerts RJ, Morris VJ
Journal:FASEB J
PubMed ID:18832596
It has been reported that modified forms of pectin possess anticancer activity. To account for this bioactivity, it has been proposed that fragments of pectin molecules can act by binding to and inhibiting the various roles of the mammalian protein galectin 3 (Gal3) in cancer progression and metastasis. Despite this ... More
Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver.
Authors:Lee SJ, Park SY, Jung MY, Bae SM, Kim IS
Journal:Blood
PubMed ID:21427291
Aged or damaged RBCs are effectively removed from the blood circulation by Kupffer cells in the liver, but little is known regarding the mechanism of the clearance process. Here we show that stabilin-1 and stabilin-2 in hepatic sinusoidal endothelial cells (HSECs) are critical in effectively clearing damaged RBCs in mouse ... More
Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy.
Authors:Gunning AP, Chambers S, Pin C, Man AL, Morris VJ, Nicoletti C
Journal:FASEB J
PubMed ID:18263697
Specific molecular-receptor interactions with gut epithelium cells are important in understanding bioactivity of food components and drugs, binding of commensal microflora, attachment and initiation of defense mechanisms against pathogenic bacteria and for development of targeted delivery systems to the gut. However, methods for probing such interactions are lacking. Methodology has ... More
7 total citations

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