Pierce™ Glycoprotein Carbohydrate Estimation Kit
Pierce™ Glycoprotein Carbohydrate Estimation Kit
Thermo Scientific™

Pierce™ Glycoprotein Carbohydrate Estimation Kit

The Thermo Scientific Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percentRead more
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Catalog NumberQuantity
23260500 mg
Catalog number 23260
Price (USD)
576.00
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500 mg
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Price (USD)
576.00
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The Thermo Scientific Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

Features of the Glycoprotein Carbohydrate Estimation Kit:

Qualitative—easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
Semi-quantitative—estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
Simple procedure—completed in less than 75 minutes; instructions include microplate and test tube protocols
Adaptable—standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (percent carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550 nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Applications
• Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents
• Characterize and classify purified protein fractions from an affinity procedure
• Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Related Products
Glycoprotein Standards Set for Pierce™ Glycoprotein Carbohydrate Estimation Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayGlycoprotein Carbohydrate Estimation
DescriptionPierce Glycoprotein Carbohydrate Estimation Kit
For Use With (Application)Solution-based Detection, Absorbance
For Use With (Equipment)Spectrophotometer, Microplate Reader
Product LinePierce™
Product TypeGlycoprotein Carbohydrate Estimation
Quantity500 mg
SpecificityNot Target-Specific
Sufficient For60 Tube Assays or 250 Microplate Assays
Detection MethodColorimetric
Unit SizeEach
Contents & Storage
Sufficient For: 60 tube assays or 250 microplate assays
• Sodium meta-Periodate, 500 mg
• Pierce Glycoprotein Detection Reagent, 500 mg
• Glycoprotein Assay Buffer, 250 mL
• Glycoprotein Standards Set, 5-protein set

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Lot #Certificate TypeDateCatalog Number(s)
ZJ395886Certificate of AnalysisNov 08, 202423260
ZC386718Certificate of AnalysisApr 07, 202423260
YJ372284Certificate of AnalysisOct 26, 202323260
YF362995Certificate of AnalysisJun 09, 202323260
XH352091Certificate of AnalysisSep 03, 202223260
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