Easy-Titer™ Rabbit IgG Assay Kit
Easy-Titer™ Rabbit IgG Assay Kit
Thermo Scientific™

Easy-Titer™ Rabbit IgG Assay Kit

The Thermo Scientific Easy-Titer Rabbit IgG Assay Kit includes antibody-sensitized microspheres to measure the specific concentration of antibodies by anRead more
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Catalog NumberQuantity
2330596 Tests
Catalog number 23305
Price (USD)
594.00
Each
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Quantity:
96 Tests
Price (USD)
594.00
Each
Add to cart
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The Thermo Scientific Easy-Titer Rabbit IgG Assay Kit includes antibody-sensitized microspheres to measure the specific concentration of antibodies by an easy and rapid microagglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). This kit is specific for rabbit IgG and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. It is sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

General features of Easy-Titer Antibody Assay Kits:

Antibody-based specificity—measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
Faster and easier than ELISA—three-component, homogenous assay; 10 minutes total incubation time
No special equipment needed—uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
Sensitive—assay range (standard curve) 8 to 500 ng/mL; use sample at 15 to 300 ng/mL for optimal results
Reproducible—coefficient of variation less than 5%; error depends on dilution and pipetting technique
Antibody standards sold separately—see product list for suggested products; use any antibody standard with proper target identity and known concentration (greater than 10 μg/mL)
Kits for five popular targets—choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody

Easy-Titer Assay Kits detect and measure specific target antibodies using agglutination of microspheres that are coated ('sensitized') with the specific anti-IgG or IgM polyclonal antibodies. In the appropriate aqueous buffer (supplied in kit), the monodispersed antibody-coated microspheres (> 1 μM diameter) have highest absorptivity (λ-max) to incident light having a wavelength (340nm) that is equal to approximately half their diameter. When sample is added, two or more microspheres bind to each antibody target via their coated specific polyclonal antibodies, and this agglutination into effectively larger apparent spheres results in proportional decrease in absorptivity (lower absorbance).

Typical microagglutination assays depend on a change in light-scattering and corresponding change in transmittance, to which absorbance is inversely related. Easy-Titer Assay Kits use a special dilution buffer whose refractive index eliminates the effect of light-scattering on the monodispersed microspheres for the measurement wavelength used. Because of this, the final 10- to 20-fold dilution of the sample for use in the assay must be done using the Dilution Buffer supplied in the kit.

Easy-Titer Antibody Assays are faster and easier than ELISA:
• Prepare standards (5 to 500 ng/mL) by diluting purified antibody in Kit Dilution Buffer.
• Prepare samples by diluting in Dilution Buffer to within assay range (8 to 500 ng/mL).
• Vortex vial of microsphere beads to create homogeneous suspension.
• Pipette 20 μL of bead suspension and 20 μL of each sample and standard into 96-well microplate wells.
• Incubate microplate for 5 minutes with vigorous mixing.
• Add 100 μL of Kit Blocking Reagent.
• Incubate microplate for 5 minutes with vigorous mixing.
• Measure absorbance on standard plate reader (340nm or 405nm).
• Plot standard curve and interpolate samples to determine concentration.

Related Products
Easy-Titer™ Mouse IgG Assay Kit
Easy-Titer™ Human IgG (H+L) Assay Kit
Easy-Titer™ Human IgG (gamma chain) Assay Kit
Easy-Titer™ Human IgM Assay Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionEasy-Titer Rabbit IgG Assay Kit
For Use With (Application)Solution-based Detection, Absorbance
For Use With (Equipment)Spectrophotometer, Microplate Reader
Quantity96 Tests
AssayProtein Quantitation Assay, Isotyping Assay, Immunoassay
Detection MethodColorimetric
Product LineEasy-Titer™
Target SpecificityTarget-specific
Unit SizeEach
Contents & Storage
Sufficient For: 96 total tests (including 9 standards per use)
• Goat Anti-Rabbit IgG Sensitized Beads, 2mL
• Easy-Titer Dilution Buffer, 30 mL
• Easy-Titer Blocking Buffer, 15 mL

Store at 4°C (do not freeze).
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Frequently asked questions (FAQs)

What do I need to consider when designing a peptide for antibody production?

We recommend that hydrophobic residues comprise 50% or less of all the residues in your sequence. Make sure there is at least one charged residue for every five amino acids: this is generally known to enhance the solubility of the peptide. Peptides (compared to polypeptides, which fold and bury the hydrophobic amino acids) are too small to fold, so just a few hydrophobic amino acids may leave them insoluble.

Other guidelines include:
Peptides containing multiple Cys, Met, and Trp can be hard to synthesize.
Some sequences are problematic in solid-phase peptide synthesis or cleavage and are best avoided; these include Asp-Pro sequences or stretches of amino acids that require bulky protecting groups on their side chains during synthesis.
Glycine is often good for antigenicity as it has only a hydrogen side chain; this allows for complete rotation.
Please note that we offer a Custom Antibody Production service (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html) that includes the use of our proprietary Antigen Profiler and Antigen Preparation tool (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html).

What is the optimal peptide length for antibody production?

Most peptide antigens range in length from 12 to 16 residues and are relatively easy to synthesize. Peptides of 9 residues or shorter have been effective antigens for antibody production, but peptides longer than 16 amino acids may contain several epitopes and form secondary structures. Peptides in excess of 18 residues begin to present more synthetic challenges. Before you synthesize your peptide, we recommend doing a BLASTP search using your peptide sequence. This is to make sure that the peptide is not homologous or identical to a sequence in a completely unrelated protein in the host animal.

Please note that we offer a Custom Antibody Production service (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html) that includes the use of our proprietary Antigen Profiler and Antigen Preparation tool (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html).

What happens if antibodies are stored improperly?

Improper storage of antibodies can lead to:
- Degradation: Loss of activity and specificity
- Aggregation: Formation of precipitates or clumps
- Reduced performance: Poor results in assays or experiments

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What do antibody pair kits contain, how many samples can I process, and how can I find a list of antibody pair kits by target?

Antibody pair kits contain capture antibody, detection antibody, recombinant standard and HRP conjugate. Each contains enough reagents to process forty 96-well plates. A list of Antibody Pair Kits (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) is available by target.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What do Reagent Sets contain, how many samples can I process, and how can I check if a Reagent Set is available for my target?

Reagent Sets contain Capture Antibody, Detection Antibody, Recombinant Standard, HRP Conjugate, TMB Substrate and Stop Solution. Each contains enough reagents to process five 96-well plates. Reagent Sets are included in the main list of ELISA (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) kits (search by “Reagent Set”).

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

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