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L-Glutamine (200 mM)
This product is manufactured in our Paisley, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Grand Island, US, is recommended for our customers in North America, Latin America, and the Asia/Pacific region. Click to view.
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Thermo Scientific™

L-Glutamine (200 mM)

Gibco™ L-glutamine is an amino acid required for cell culture. L-glutamine participates in the formation of purine and pyrimidine nucleotides,Read more
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Catalog NumberQuantity
2503003220 mL
25030024
also known as 25030-024
100 mL
Catalog number 25030032
Price (USD)
-
Quantity:
20 mL
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Gibco™ L-glutamine is an amino acid required for cell culture. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production. L-Glutamine, unlike most other amino acids, is not stable in solution. The rate at which this degradation proceeds is a function of time, temperature and pH. We also offer Gibco™ GlutaMAX™ supplement as a stable alternative to L-glutamine.

Gibco™ L-glutamine 200 mM Solution is a stock solution ready-to-use as a cell culture supplement. The optimal use concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2 – 6 mM.

Dual-site cGMP Manufacturing and Quality System
Gibco™ L-glutamine is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ L-glutamine product made in our Grand Island facility (25030-164). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Specifications
pH6 to 8
Recommended StorageStorage conditions: -5 to -20°C. Protect from light.
Shipping conditions: Frozen
Shelf life: 18 months from date of manufacture
Quantity20 mL
Concentration or Composition (by Analyte or Components)100 X
GlutamineL-Glutamine
Physical FormLiquid
Shipping ConditionDry Ice
Unit SizeEach

Frequently asked questions (FAQs)

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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