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Gibco™
L-Glutamine (200 mM)
Gibco L-Glutamine is a ready-to-use amino acid supplement for cell culture. L-glutamine is an amino acid required in cell culture for a variety of critical cellular processes.
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| Catalog Number | Quantity |
|---|---|
| 25030081 | 100 mL |
| 25030149 | 20 mL |
| 25030164 | 20 x 100 mL |
Catalog number 25030081
Price (USD)
37.65
Online Exclusive
40.88Save 3.23 (8%)
Each
Quantity:
100 mL
Price (USD)
37.65
Online Exclusive
40.88Save 3.23 (8%)
Each
Gibco L-Glutamine is a ready-to-use amino acid supplement for cell culture. L-glutamine is an amino acid required in cell culture for the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution with a rate of degradation dependent upon time, temperature, and pH. The optimal concentration depends upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.
Gibco L-Glutamine supplement is a ready-to-use 200 mM stock solution for cell culture supplementation that supports of many critical cellular functions, including:
- Formation of purine and pyrimidine nucleotides
- Formation of amino sugars, glutathione, and L-glutamate
- Formation of other amino acids
- Protein synthesis
- Glucose production
cGMP manufacturing and quality system
For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
Specifications
Chemical Name or MaterialGlutamine
Concentration or Composition (by Analyte or Components)100X
Green FeaturesSustainable packaging
Physical FormLiquid
Product LineGibco™
Recommended StorageStorage conditions: -5°C to -20°C. Protect from light.
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture
Shipping conditions: Dry ice
Shelf life: 24 months from date of manufacture
Shelf Life24 Months
Shipping ConditionDry Ice
Quantity100 mL
pH5 to 6
Unit SizeEach
Frequently asked questions (FAQs)
Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?
When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?
L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.
Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?
If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
What is your recommended method for thawing L-Glutamine before adding to cell culture media?
We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Why did my 200 mM L-glutamine precipitate when I thawed it?
When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.