Spectra™ Multicolor Broad Range Protein Ladder
Spectra™ Multicolor Broad Range Protein Ladder
Spectra™ Multicolor Broad Range Protein Ladder
Spectra™ Multicolor Broad Range Protein Ladder
Spectra™ Multicolor Broad Range Protein Ladder
Spectra™ Multicolor Broad Range Protein Ladder
Spectra™ Multicolor Broad Range Protein Ladder
Thermo Scientific™

Spectra™ Multicolor Broad Range Protein Ladder

Die Thermo Scientific Spectra Mehrfarbige Breitband-Proteinleiter ist ein 4-Farben-Proteinstandard (blau, orange, grün, rosa) mit 10 vorgefärbten Proteinen (10 bis 260Weitere Informationen
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KatalognummerMenge
2662310 x 250 μl
266342 x 250 μl
26634X48 x 250 µl
Katalognummer 26623
Preis (EUR)
874,00
Each
-
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Menge:
10 x 250 μl
Recurring order eligible. Learn more »
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
874,00
Each
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Ask our AI about this Product
Die Thermo Scientific Spectra Mehrfarbige Breitband-Proteinleiter ist ein 4-Farben-Proteinstandard (blau, orange, grün, rosa) mit 10 vorgefärbten Proteinen (10 bis 260 kDa) zur Verwendung in der Gel-Elektrophorese und bei Western Blots. Die Proteinleiter wird in einem gebrauchsfertigen Format geliefert zum direkten Auftragen auf Gele. Vor der Verwendung muss kein Probenpuffer erwärmt, reduziert oder hinzugefügt werden.

Alle anderen Proteinstandards und -leitern anzeigen und vergleichen ›

Anwendungen
• Überwachen der Proteinmigration während der SDS-Polyacrylamid-Gelelektrophorese
• Überwachen der Proteinübertragung auf Membranen nach Western Blotting
• Größenbestimmung von Proteinen in SDS-Polyacrylamid-Gelen und Western Blots

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
NachweisverfahrenKolorimetrisch, NIR-Fluoreszenz (700 nm), RGB-Fluoreszenz (555 nm)
GelkompatibilitätBolt™ Bis-Tris Plus Gele, Novex™ Tricin-Gele, Novex™ Tris-Glycin-Gele, NuPAGE™ Bis-Tris-Gele, NuPAGE™ Tris-Acetat-Gele, SDS-PAGE-Gele
Molekulargewicht260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa
ProduktlinieSpectra™
ProdukttypProteinleiter
Menge10 x 250 μl
Sofort einsatzbereitJa
VersandbedingungZugelassen für den Versand auf Nass- oder Trockeneis
Färbemitteltyp4 Farben: Blau, orange, grün, rosa
SystemtypWestern Blotting, SDS-PAGE
Number of Markers10
Größenbereich10 bis 260 kDa
Unit SizeEach
Inhalt und Lagerung
Inhalt: 10 250 µl-Fläschchen

Lagerpuffer: 62,5 mmol Tris-H3PO4 (pH 7,5 bei 25 °C), 1 mmol EDTA, 2 % SDS, 10 mmol DTT, 1 mmol NaN3 und 33 % Glycerin

Lagerung: Nach Erhalt bei –20 °C lagern

iBlot 3 Western Blot Transfer System

Introducing iBlot 3 Western Blot Transfer System

Featuring higher throughput and built-in cooling for consistent protein transfer
Learn more

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Zertifikate

Chargen-Nr.Certificate TypeDateCatalog Number(s)
3259871Certificate of Analysis12. Juni 202526634
3247378Certificate of Analysis27. Mai 202526623
3241359Certificate of Analysis19. Mai 202526634
3201591Certificate of Analysis01. Apr. 202526623
3198658Certificate of Analysis27. März 202526634
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Sicherheitsdatenblätter

Häufig gestellte Fragen (FAQ)

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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