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Sodium Dodecyl Sulfate (SDS), C12
Sodium Dodecyl Sulfate (SDS), C12
Thermo Scientific™

Sodium Dodecyl Sulfate (SDS), C12

Thermo Scientific Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experimentsRead more
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Catalog NumberQuantity
28312500 g
Catalog number 28312
Price (USD)
261.65
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294.00
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500 g
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Price (USD)
261.65
Online Exclusive
294.00
Save 32.35 (11%)
Each
Add to cart
Thermo Scientific Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters.

Features of Sodium Dodecyl Sulfate (C12):

• Popular anionic detergent for protein electrophoresis and solubilization
• Component of many cell lysis buffer formulations
• Specially purified formulation is >98% C12 alkyl sulfate
• Contains low levels of hexadecyl sulfate (C16), which inhibits protein renaturation

Sodium dodecyl sulfate (SDS) is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. It is a component of many cell lysis buffer formulations, including RIPA buffer. Typical SDS or lauryl sulfate preparations are mixtures of several different chain lengths (C10 to C18). However, protein renaturation and other specialized methods require very specific surfactant and buffer conditions. This particular formulation of SDS is highly purified to eliminate nearly all secondary chain lengths besides C12.

Properties of Sodium Dodecyl Sulfate, (pure C12):
• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8 mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate (SDS), C12 (Part No. 28312):
• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless and free of particulates.
• Chain length: C12 ≥98.0% if C16 ≤0.8%
• Report C10, C12, C14, C16, C18 concentrations.

Related Products
Sodium Dodecyl Sulfate (SDS), Lauryl
Sodium Dodecyl Sulfate (SDS), 20% Solution
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity500 g
Reagent TypeDetergent (Pure)
FormPowder
Product TypeDetergent
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20°C to 25°C.

Frequently asked questions (FAQs)

Are detergents denaturing or non-denaturing with respect to protein structure?

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).



Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are detergents?

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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