Tween™ 80 Surfact-Amps™ Detergent Solution
Tween™ 80 Surfact-Amps™ Detergent Solution
Thermo Scientific™

Tween™ 80 Surfact-Amps™ Detergent Solution

Thermo Scientific Tween-80 Surfact-Amps Detergent Solution is a highly-purified Tween™ 80 detergent stabilized as a 10% solution (w/v) and packagedRead more
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Catalog NumberQuantity
2832950 mL
283286 x 10 mL
28230500 mL
Catalog number 28329
Price (USD)
151.65
Online Exclusive
180.00
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50 mL
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Price (USD)
151.65
Online Exclusive
180.00
Save 28.35 (16%)
Each
Add to cart
Thermo Scientific Tween-80 Surfact-Amps Detergent Solution is a highly-purified Tween™ 80 detergent stabilized as a 10% solution (w/v) and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring it's stability and eliminating the accumulation of peroxides and degradation products.

Features of Tween-80 Surfact-Amps Detergent Solution:

Tween 80—a popular nonionic detergent for use in ELISA, Western blotting and other immunoassay PBS or TBS wash buffers
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0μeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in glass ampules or HDPE bottles

Properties of Tween 80:
• Molecular Weight: 1310g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: 60
• Micelle Molecular Weight: 76,000g
• Critical Micelle Concentration (CMC): 0.012 mM (0.0016%, w/v)
• Cloud Point: Unknown
• Dialyzable: No

Specifications for Tween 80 Surfact-Amps Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0μeq/mL
• Carbonyls: ≤1.0μeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Related Products
Surfact-Amps™ Detergent Sampler
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity50 mL
Reagent TypeDetergent Solution
FormLiquid
Product LineSurfact-Amps™, Tween™
Product TypeDetergent
Unit SizeEach
Contents & Storage
Store in a cool, dry, and well-ventilated area

Frequently asked questions (FAQs)

What are the main advantages of using Thermo Scientific Pierce Surfact-Amps detergents?

Thermo Scientific Pierce Surfact-Amps detergents are highly purified, precisely diluted (10%) formulations that are ideal for applications or assays that are sensitive to contaminants that are present in unpurified detergents. We test every batch to insure that our detergents contain less than 1.0 µeq/mL peroxides and carbonyls and package them under nitrogen, to prevent oxidization during storage.

Superior quality - lower measurable contaminant levels than other leading vendors
Accurate -precise 10% detergent solution in ultrapure water
Easy-to-use - solution is simple to dispense and dilute for use
Exceptionally pure - less than 1.0 µeq/mL peroxides and carbonyls

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Are detergents denaturing or non-denaturing with respect to protein structure?

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).



Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are detergents?

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does detergent-based cell lysis work?

Detergents are amphipathic molecules, meaning they contain both a nonpolar “tail” having aliphatic or aromatic character and a polar “head”. Like the components of biological membranes, detergents have hydrophobic-associating properties as a result of their nonpolar tail groups. Nevertheless, detergents are themselves water soluble.

Consequently, detergent molecules allow the dispersion (miscibility) of water-insoluble, hydrophobic compounds into aqueous media, including the extraction and solubilization of membrane proteins. Detergent monomers solubilize membrane proteins by partitioning into the membrane bilayer. With increasing amounts of detergents, membranes undergo various stages of solubilization.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What types of detergents are available for cell lysis?

Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking proteinprotein interaction. These detergents are considered harsh. Non-denaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS). These detergents do not denature proteins and do not break protein-protein interactions. These detergents are considered mild.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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