Medium 199, Earle's Salts
This product is manufactured in our Paisley, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Grand Island, US, is recommended for our customers in North America, Latin America, and the Asia/Pacific region. Click to view.
Medium 199, Earle's Salts
Gibco™

Medium 199, Earle's Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivationRead more
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Catalog NumberQuantity
31150022500 mL
11150059500 mL
1115006710 x 500 mL
Catalog number 31150022
Price (USD)
-
Quantity:
500 mL
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Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This Medium 199 is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red 


The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell TypeMammalian Cells
Concentration1X
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality290 - 310 mOsm/kg
Product LineGibco™
Product TypeMedium 199
Quantity500 mL
Shelf Life12 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
Culture TypeMammalian Cell Culture
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red, Earle's Balanced Salt Solution (EBSS)
Without AdditivesNo HEPES, No Sodium Pyruvate, No Sodium Pyruvate, No Sodium Bicarbonate
pH7.1 to 7.4
Unit SizeEach
Contents & Storage
Storage conditions: 2–8°C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Media Formulations

Frequently asked questions (FAQs)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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