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引用資料與參考文獻 (2)
Gibco™
MEM α, GlutaMAX™ Supplement, no nucleosides
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative深入閱讀
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變更視圖
| 產品號碼 | Quantity |
|---|---|
| 32561037 | 500 mL |
| 32561102 | 10 x 500 mL |
產品號碼 32561037
價格 (TWD)
***
Quantity:
500 mL
價格 (TWD)
***
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
| With | Without |
| • Phenol Red | • Ribonucleosides |
| • GlutaMAX™ | • Deoxyribonucleosides |
The complete formulation is available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Gibco™ MEM α with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
規格
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™, GlutaMAX™
Product TypeMEM α (Minimum Essential Medium α)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesLow Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES, No Ribonucleosides, No Deoxyribonucleosides
Unit SizeEach
內容物與存放
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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圖表
L-glutamine is prone to spontaneous degradation
L-glutamine degrades faster than GlutaMAX Supplement
GlutaMAX Supplement delivers L-glutamine with more stability
GlutaMAX Supplement reduces ammonia production from L-glutamine
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證書
依批號或部分批號搜尋
批號Certificate TypeDateCatalog Number(s)
3166021Certificate of AnalysisJun 27, 202532561029, 32561037
3224747Certificate of AnalysisMay 31, 202532561029, 32561037
3090801Certificate of AnalysisApr 04, 202532561029, 32561037
3042527Certificate of AnalysisJan 31, 202532561029, 32561037
2537228Certificate of AnalysisJan 29, 202532561029, 32561037
顯示 5 個結果, 在上方搜尋特定憑證
安全資料表
SDS常見問答集 (常見問題)
The osmolality is listed in the COA for the particular lot number of the medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
引用資料與參考文獻 (2)
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引用資料與參考文獻
Abstract
Point mutations identified in Lec8 Chinese hamster ovary glycosylation mutants that inactivate both the UDP-galactose and CMP-sialic acid transporters.
Journal:J Biol Chem
PubMed ID:11319223
'Nucleotide-sugar transporters (NSTs) are critical components of glycosylation pathways in eukaryotes. The identification of structural elements that are involved in NST functions provides an important task. Chinese hamster ovary glycosylation mutants defective in nucleotide-sugar transport provide access to inactive transporters that can define such structure/function relationships. In this study, we
Isolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries.
Journal:Nat Protoc
PubMed ID:23598447
Accruing evidence indicates that production of new oocytes (oogenesis) and their enclosure by somatic cells (folliculogenesis) are processes not limited to the perinatal period in mammals. Endpoints ranging from oocyte counts to genetic lineage tracing and transplantation experiments support a paradigm shift in reproductive biology involving active renewal of oocyte-containing
2 total citations