AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

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AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl<sub>2</sub>

Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

Q: There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a ball-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

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Catalog Number	Quantity
4311806	250 Units
4311816	1000 Units
4311820	3000 Units
4311818	5000 Units
4317742	25,000 Units

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Catalog number 4311806

Price (USD)

335.65

Online Exclusive

357.00

Save 21.35 (6%)

Each

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Quantity:

250 Units

Request bulk or custom format

Price (USD)

335.65

Online Exclusive

357.00

Save 21.35 (6%)

Each

Add to cart

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Features

Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
Time-released thermal activation improves sensitivity in low copy number amplifications
Successful multiplex PCR saves time and reagents
Includes GeneAmp 10X PCR Gold Buffer with MgCl₂

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold DNA Polymerase is a chemical hot-start enzyme, there is limited risk of biological contamination.

GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

Notes

AmpliTaq Gold 360 DNA Polymerase is available for even better PCR performance.
AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration5 U/μL

Exonuclease Activity5' - 3'

Fidelity (vs. Taq)1X

FormatTube

Hot StartBuilt-In Hot Start

No. of Reactions200 Reactions

Overhang3'-A

PolymeraseAmpliTaq Gold DNA Polymerase

Product TypeDNA Polymerase

Quantity250 Units

Reaction FormatSeparate Components

Shipping ConditionDry Ice

Size (Final Product)5 kb or less

Starting MaterialDNA

For Use With (Application)Hot-start PCR

GC-Rich PCR PerformanceLow

Reaction SpeedStandard

Unit SizeEach

Contents & Storage

• AmpliTaq Gold DNA Polymerase (5 U/μL), 1 x 50 μL
• GeneAmp 10X Gold Buffer, 1 x 1.5 mL
• 25 mM MgCl₂, 1 x 1.5 mL

Store at -15°C to -30°C.

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Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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