POP-7™ Polymer for 3730/3730xl DNA Analyzers
New Capillary Electrophoresis Instruments Support Center—Find technical tips and troubleshooting recommendations from our scientists.
Product Image
Applied Biosystems™

POP-7™ Polymer for 3730/3730xl DNA Analyzers

POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencingRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
43639291 x 28 mL
43356155 x 28 mL
436393510 x 28 mL
433561130 x 28 mL
Catalog number 4363929
Price (USD)
1,836.00
1 x 28 mL
In stock
Add to cart
Quantity:
1 x 28 mL
Price (USD)
1,836.00
1 x 28 mL
Add to cart
POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis. Not suitable for use with ABI PRISM ™, 310 and 3100 series genetic analyzers. It causes syringe failure and premature array degradation. Sufficient for 8,000 - 12,000 samples.

POP-7™ Polymers for the 3730⁄3730xl Genetic Analyzers is:
• Pre-formulated, which not only saves time but also saves reproducibility by ensuring consistent formulation
• Formulated for sequencing and fragment analysis applications
• Designed to allow capillaries to be used multiple times

Reproducible Results
POP™ polymers dynamically coat the capillary wall to control electro-osmotic flow. Their definite quality and uniform consistency eliminate guesswork and help to ensure reproducibility. POP polymers are specifically formulated to separate DNA fragments of a known size range at a desired resolution and run time.

Used With
3730/3730xl Genetic Analyzer Capillary Array, 50 cm, 3730 Running Buffer, 10X, BigDye™ Terminator v1.1 Cycle Sequencing Kit, BigDye™ Terminator v3.1 Cycle Sequencing Kit, BigDye™ Terminator v3.1 Cycle Sequencing Kit, BigDye™ Terminator v3.1 Cycle Sequencing Kit, Hi-Di™ Formamide.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accuracy98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary)
For Use With (Application)Standard Sequencing, Fragment Analysis (Denaturing)
For Use With (Equipment)3730xl DNA Analyzer, 3730 DNA Analyzer
On Instrument Life UsageStability on instrument 7 days, sufficient for 8000 to 12,000 samples
Product LinePOP-7™
Product TypePolymer
Quantity1 x 28 mL
Test Time1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.)
Unit Size1 x 28 mL
Contents & Storage
Refrigerate at 2°C to 8°C.

Frequently asked questions (FAQs)

How many samples can I run with one 28 ml bottle of POP-7 polymer on my Applied Biosystems 3730 Series DNA Analyzer?

You can run 8,000-12,000 samples. These numbers may be lower if there is increased polymer consumption due to additional wizards being performed, such as the Bubble Remove and Water Wash wizards.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

How long can POP-7 polymer stay on the Applied Biosystems 3730 Series DNA Analyzer?

POP-7 polymer can maintain its performance for seven days on the Applied Biosystems 3730 Series DNA Analyzer at standard lab conditions. After seven days, perform all required maintenance (including the Water Wash Wizard) and replace with fresh polymer. Do not "top off" the polymer bottle (add fresh polymer to old polymer), which can adversely affect data quality and consumable lifespan.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

What polymer is supported on the Applied Biosystems 3730 Series DNA Analyzer?

Applied Biosystems supports the use of POP-7 polymer on the Applied Biosystems 3730 Series DNA Analyzer. If the instrument is running Applied Biosystems 3730 Data Collection Software v.3.1 or later, then you can also use POP-6 polymer. We do not recommend mixing and matching of various instrument polymers as each is manufactured in different processes, tested as an entire system, and guaranteed to perform to a minimum specification. Substitution of reagents may compromise the integrity of the system and cannot be supported by AB service or applications support specialists. Using unsupported polymers may adversely affect data quality, consumable lifespan, and may void existing warranties.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Does the Applied Biosystems 3130 Series use the same POP-7 Polymer as the Applied Biosystems 3730 Series?

Yes, Applied Biosystems 3130 Series Systems use the same POP-7 Polymer as Applied Biosystems 3730 series instruments, but is sold in a convenient 7 mL volume bottle which is aligned with expected Applied Biosystems 3130 Series throughput. We do not recommend customers purchase the Applied Biosystems 3730 series sized POP-7 Polymer due to the difference in throughput, limited shelf life and potential problems with pouring.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

What is the white residue around the array knob on my Applied Biosystems 3730/3730xl DNA Analyzer?

When placing the array on the instrument, the last step in the process is to loosen the array knob and the instrument forces any air bubbles around the array tip through the threading and the middle of the array knob. Some polymer gets pushed through as well and when it dries up, it appears as a white, flaky residue. Use a damp, lint-free cloth to remove it. If more residue appears after the cleanup, it is possible that the array knob is not tightened enough or the array ferrule may require replacement.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link

1x1 image pixel for data collection