7500 Real-Time PCR System for Human Identification with Dell Notebook D520
7500 Real-Time PCR System for Human Identification with Dell Notebook D520
Applied Biosystems™

7500 Real-Time PCR System for Human Identification with Dell Notebook D520

The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternityRead more
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Catalog NumberQuantity
43666051 system
Catalog number 4366605
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The Applied Biosystems™ 7500 Real-Time PCR System for Human Identification provides an advanced, validated solution for casework, databasing, and paternity applications. This system comes with a Dell Notebook D520 computer.

This instrument system provides the following:
• It is validated for use with the Quantifiler™ Human Quantification and the Quantifiler™ Y Human Male DNA Quantification Systems.
• It utilizes the Quantifiler™ kits to determine the quantity of amplifiable DNA and the potential presence of PCR inhibitors to obtain an accurate prediction of the amount of DNA input required for optimal STR amplification.
• It utilizes the Quantifiler ™ kits to quantify trace samples; real-time PCR has the widest dynamic range of any DNA detection technology.
• It provides Quantfiler™ kit results in less than 2 hours.

Built for Performance
The 7500 system for Human Identification includes SDS v.1.2.3 software which is validated to run Quantifiler™ Human DNA and Quantifiler™ Y Human Male DNA quantification kits. SDS v1.2.3 is a powerful, versatile software that includes plate set-up wizards and automated analysis tools that make data processing easier. Its advanced data viewing capabilities make analysis less time consuming. The instrument is a powerful, five-color platform that is calibrated for the broadest range of dyes available: FAM™⁄SYBR™ Green I, VIC™⁄JOE™, TAMRA™⁄CY3, ROX™⁄Texas Red, and CY5 dyes. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets and its advanced multi-componenting algorithm minimizes spectral crosstalk making the instrument superior for multiplexing.

Complete Validated Solution
Applied Biosystems™ offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR™ STR kits, instrumentation, software, and consumables. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD. The system has been internally validated by Applied Biosystems™ following DAB⁄SWGDAM guidelines for use with Quantifiler™ Human Quantification System and Quantifiler™ Y Human Male DNA Quantification System making the 7500 system suitable for casework, paternity, and databasing laboratories.

EXPERIENCE MATTERS™
When you partner with Applied Biosystems™ you gain access to the extensive knowledge and experience of our forensic support team and training resources dedicated to Human Identification laboratories. Our HID University™ provides continuing education for DNA analysts, our support and Applications Scientists offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibrated DyeSYBR™ Green I, ROX™, Texas Red™, NED™, JOE™, Cy3™, Cy5™, VIC™, FAM™, TAMRA™
DimensionsWidth 34 cm (13.39 in.), Depth 45 cm (17.72 in.), Height 49 cm (19.29 in.)
Dynamic Range9 Logs
Electrical Requirements950 W
External ComputerDell Notebook D520
For Use With (Equipment)7500 System
Format96-well Plate
High-throughput CompatibilityMultiplexing
OpticsCCD Camera, 5 Excitation Filters, Tungsten-halogen Lamp, 5 Emission Filters
Passive Reference DyeROX (Separate Tube), No ROX, ROX (Pre-mixed)
Peak Block Ramp Rate2.5°C⁄s
Precision99.7% Confidence (5000 & 10000 Copies)
Quantity1 system
Reaction SpeedStandard
Reaction Volume Range20-100 μl
Sample Ramp Rate± 1.1°C⁄s
Sensitivity1 Copy of RNase P gene (Human Genomic DNA)
Software CompatibilityGeneMapper™ ID v3.2, 7500 System Software, HID Real Time PCR Analysis Software v1.1
Temperature Range (Metric)4 to 100°C
Thermal Accuracy0.25°C (35°C to 95°C after 3 min.)
Thermal Cycling SystemPeltier-based System
Thermal Uniformity0.50°C (after 30 s)
Throughput4 to 5-96-well Plates per 8-hr day
Voltage120 V
Weight75 lb.
Unit SizeEach
Contents & Storage
Ambient

Frequently asked questions (FAQs)

What run conditions should I use for the TaqMan® Advanced miRNA assays with the discontinued 7900HT Real-Time PCR System?

We recommend using the same thermal profile as listed for the 7500 and 7500 Fast systems (Table 8) in the TaqMan® Advanced miRNA Assays User Guide: https://assets.thermofisher.com/TFS-Assets%2FLSG%2Fmanuals%2F100027897_TaqManAdv_miRNA_Assays_UG.pdf

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