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Applied Biosystems™
High-Capacity cDNA 反转录试剂盒
The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from了解更多信息
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生命科学满额赠礼，10款热门试剂0元任选
6/17-6/30，购生命科学飞享价产品单笔满5千赠1件，满1万赠3件热门试剂更多
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| 货号 | 包括 | 反应次数 |
|---|---|---|
| 4368814 | Kit only | 200 次反应 |
| 4374966 | Kit with Rnase Inhibitor | 200 次反应 |
| 4368813 | Kit only | 1,000 次反应 |
| 4374967 | Kit with Rnase Inhibitor | 1,000 次反应 |
货号 4368814
价格（CNY)
2,615.00
飞享价
Ends: 31-Dec-2025
4,448.00共减 1,833.00 (41%)
Each
有货
包括:
Kit only
反应次数:
200 次反应
物流信息
常规产品： 发货后, 北上广深及省会城市通常为 1-2天,二线城市通常 2-3天,三线以及偏远地区通常 4天，周末以及节假日顺延。
空运受限产品： 发货后, 北上广深及省会城市通常为 2-4天,二线城市 通常3-5天,三线以及偏远地区通常 6-10天，周末以及节假日顺延。
现货中心： 此类产品将由您身边的现货中心极速配送。
空运受限产品： 发货后, 北上广深及省会城市通常为 2-4天,二线城市 通常3-5天,三线以及偏远地区通常 6-10天，周末以及节假日顺延。
现货中心： 此类产品将由您身边的现货中心极速配送。
价格（CNY)
2,615.00
飞享价
Ends: 31-Dec-2025
4,448.00共减 1,833.00 (41%)
Each
The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 μg input total RNA. Ideal for generating cDNA archives. The High Capacity cDNA Reverse Transcription Kit can convert up to 2 μg of input total RNA in a single 20 μl reaction. Reactions can be scaled up to 100 μl to generate 10 μg of cDNA from a single reaction The cDNA generated is ideal for a range of applications including real-time PCR, standard PCR, and microarrays.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
仅供科研使用。不可用于诊断程序。
规格
浓度50 U/μL
最终产品类型第一链 cDNA
产品规格试剂盒
包括Kit only
反应次数200 次反应
最佳反应温度37°C
数量200 次反应
反应形式单独组分
试剂类型反转录
逆转录酶MultiScribe™
核糖核酸酶 H 活性是
运输条件干冰
尺寸（最终产品）长达 7 kb
原始材料RNA
技术反转录
适用于（应用）实时 PCR (qPCR)
反应速度120 min。
Unit SizeEach
内容与储存
• 10X RT 缓冲液，1 mL
• 10X RT 随机引物，1 mL
• 25X dNTP 混合物，0.2 mL (100 mM)
• MultiScribe 逆转录酶，0.1 mL (50 U/μL)
储存在 –25°C 至 –15°C 下。
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证书
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批号Certificate TypeDateCatalog Number(s)
3270174Certificate of Analysis2025年6月27日4368814
3269125Certificate of Analysis2025年6月26日4374966
3267491Certificate of Analysis2025年6月25日4374966
3267502Certificate of Analysis2025年6月25日4374966
3262537Certificate of Analysis2025年6月17日4368813
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SDS常见问题解答 (FAQ)
You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.
Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.
We recommend the following kits:
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
It contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).
引用和文献 (1122)
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引用和文献
Abstract
Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.
Journal:The Journal of biological chemistry
PubMed ID:18077444
Increased hepatobiliary clearance of unconjugated thyroxine determines DMP 904-induced alterations in thyroid hormone homeostasis in rats.
Journal:Toxicological sciences : an official journal of the Society of Toxicology
PubMed ID:15673846
4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyri midine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell
Somatic cell plasticity and Niemann-Pick type C2 protein: fibroblast activation.
Journal:J Biol Chem
PubMed ID:21084287
A growing body of evidence points toward activated fibroblasts, also known as myofibroblasts, as one of the leading mediators in several major human pathologies including proliferative fibrotic disorders, invasive tumor growth, rheumatoid arthritis, and atherosclerosis. Niemann-Pick Type C2 (NPC2) protein has been recently identified as a product of the second
TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4.
Journal:J Biol Chem
PubMed ID:21324893
MicroRNAs (miRs) are emerging as prominent players in the regulation of many biological processes, including myogenic commitment and skeletal muscle formation. Members of the TGF-beta family can influence the proliferation and myogenic differentiation of cells, although it is presently not clear what role miRNAs play in the TGF-beta-mediated control of
Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.
Journal:J Exp Med
PubMed ID:19652016
The cause of Crohn's disease (CD) remains poorly understood. Counterintuitively, these patients possess an impaired acute inflammatory response, which could result in delayed clearance of bacteria penetrating the lining of the bowel and predispose to granuloma formation and chronicity. We tested this hypothesis in human subjects by monitoring responses to
1122 total citations