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MicroSEQ™ Listeria monocytogenes Detection Kit
MicroSEQ&trade; <i>Listeria monocytogenes</i> Detection Kit
Applied Biosystems™

MicroSEQ™ Listeria monocytogenes Detection Kit

This kit is recommended for users who have already purchased the starter kit but would like to perform more tests.Read more
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Catalog NumberQuantity
44038741 kit
Catalog number 4403874
Price (USD)
1,456.00
Each
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Quantity:
1 kit
Recurring order eligible. Learn more »
Price (USD)
1,456.00
Each
Add to cart
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This kit is recommended for users who have already purchased the starter kit but would like to perform more tests. The MicroSEQ™ Listeria monocytogenes Detection Kit detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan™ probe to detect the amplified sequence.
• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total)
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ™ Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps:
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ™ Listeria monocytogenes Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 cfu per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ™ Listeria monocytogenes Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.
Specifications
Detection MethodPrimer-Probe Detection
Hot StartBuilt-In Hot Start
PCR MethodqPCR
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq™ Fast DNA Polymerase
Primer Probe CompatibilityTaqMan™
Product LineMicroSEQ™
Quantity1 kit
Sample TypeAny Swab Type, Food, Water Samples
Target Organism ClassL. monocytogenes
Test Time< 3 hr.
TypeListeria Test Kit
Validated ApplicationReal-Time PCR
Unit SizeEach
Contents & Storage
This kit includes 12 8-tube strips containing assay beads (store at 2 to 8°C in the dark), optical 8-tube strips and 8-cap strips (store at 22 to 28°C), and a Negative Control (store at 2 to 8°C).

These reagents are sufficient for 96 reactions.
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Safety Data Sheets

Limited Use Label Licenses (LULL)

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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product (a) to perform internal research for the sole benefit of the purchaser; and (b) for environmental testing, quality control/quality assurance testing, food and agricultural testing, including reporting results of purchaser's activities in environmental testing, quality control/quality assurance testing, food and agricultural testing for a fee or other commercial consideration. No other right is hereby granted expressly, by implication, or by estoppel. This product is for environmental testing, quality control/ quality assurance testing, food and agricultural testing and research purposes only. The purchase of this product does not grant the purchaser any additional rights, including (without limitation) the right to transfer or resell the product in any form or the right to use the product as a therapeutic agent or diagnostics test component. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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Frequently asked questions (FAQs)

Without performing any specific pre-enrichment step on certain food samples like meat, the use of the PrepMan Ultra Sample Preparation Reagent (P/N 4318930) will not enable the reduction of many forms of PCR inhibitors such as fats, tissues and particulates. Thus, pre-enrichment significantly reduces the detection of false positive signal from dead or nonpathogenic cells in food samples. Improper pre-enrichment steps can hamper the process of extracting DNA of the highest purity and affect the performance of TaqMan Pathogen Detections kits for Salmonella enterica, Listeria monocytogenes, Campylobacter jejuni, E. coli 0157:H7, or MicroSeq kits.

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