TaqMan™ Array Human NFKB Pathway
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TaqMan™ Array Human NFKB Pathway
Applied Biosystems™

TaqMan™ Array Human NFKB Pathway

The Applied Biosystems™ TaqMan™ Array Human NFkB Pathway 96-well Plate contains 92 assays to NFKB associated genes and 4 assaysRead more
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Catalog NumberQuantity
44140951 standard plate
Catalog number 4414095
Price (USD)
262.00
Each
Estimated availability date 15-Jul-2025
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Quantity:
1 standard plate
Price (USD)
262.00
Each
Add to cart
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The Applied Biosystems™ TaqMan™ Array Human NFkB Pathway 96-well Plate contains 92 assays to NFKB associated genes and 4 assays to candidate endogenous control genes.

The panel of assays in this plate targets genes which encode the 5 proteins of the Rel/NFKB family: NFKB1, NFKB2, REL A, REL B, and REL. Also on the panel are genes from families that include: the IkB kinase; IkBs (inhibitor of kB); Toll-like receptor (TLR); Tumor Necrosis Factor (TNF); Tumor Necrosios Factor Receptors (TNFR); and Tumor Necrosis Factor Receptor Associated Factor (TRAF). Additional genes associated with NFKB function in apoptosis, immune/inflammation responses, as well as chemokines and cytokines are included. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan™ Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan™ probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan™Assay Quality, New Format

TaqMan™ Array Plates contain the same high quality TaqMan™ Gene Expression Assays (PCR primers and TaqMan™ probe sets) that have been commercially available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan™Assays supplied in the traditional liquid format and assays dried into a TaqMan™ Array Plate. Figure 1 shows the results of an experiment comparing the linear range of the two formats, where no difference in performance was observed.

Just Add Master Mix and Sample, and Begin Cycling
Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include just the right amount of TaqMan™Assay in each well: enough for one 20 μl reaction. You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased TaqMan™Assays. Save a significant amount of time setting up your experiments. Just add master mix and sample, and begin cycling.

Figure 2 is an example Plate Layout with Gene Symbols and Assay IDs

Plate Map Ensures Proper Data Labeling and Easy Re-Ordering
Each pack of TaqMan™ Array Plates is shipped with a CD containing the plate configuration map (see Figure 2) along with the details of each TaqMan™Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.

Simple to Use
Each TaqMan™ Array Plate well contains a single TaqMan™Assay for one 20 μl reaction. The plates can be stored at room temperature or at 4°C. When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument.

Compatibility
TaqMan™ Array Plates have been optimized for use with TaqMan™ Gene Expression Master Mix, but are also compatible with TaqMan™ Universal PCR Master Mix. For studies using cultured cells, TaqMan™ Array Plates are also compatible (and recommended) with the TaqMan™ Gene Expression Cells-to-Ct™ Kit. TaqMan™ Array Plates must be run on a real-time PCR instrument with 96-well plate capability. The plates have been validated for use on Applied Biosystems™ 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 μl reaction
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Format96-well plate
Internal Probe ModificationMGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
No. of Reactions1 x 20 μL Reactions
Product LineTaqMan™
Purification MethodStandard
Quantity1 standard plate
Research CategoryCell Cycle & Proliferation, Apoptosis, Neurobiology, Biomarker-Related Pathway(s), Oncology, Development, Immunology, Signal Transduction
SpeciesHuman
FormDried Down
Label or DyeFAM
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Each TaqMan™ Array Plate well contains a single TaqMan™ Assay for one 20 μL reaction. The plates can be stored at room temperature or at 4°C.
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Frequently asked questions (FAQs)

What is the minimum amount of TaqMan array plates I need to purchase to submit an order?

The minimum amount of TaqMan Array plates needed to submit an order is based on the plate type:
• Inventoried TaqMan array plates: 1 plate
• TaqMan Flexible Content panel array plates: 6 plates
• Custom TaqMan array plates: 6 plates
• Specialty TaqMan array plates: 10-20 plates (depends on assay format and configuration). Please contact our Specialty Plates team at Specialty_Plates@thermofisher.com for details.

Can our TaqMan Array Plates be run on non-Applied Biosystem real-time PCR instruments?

We recommend using our Plastics Selection Tool to verify which plates are compatible with your instrument model, https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html.

Based on our testing, the 96-well TaqMan Array Plates can be used on the following non-Applied Biosystem real-time PCR instruments:
• Standard (0.2mL) 96-well Plates: Bio-Rad IQ5
• Fast (0.1mL) Plates: Bio-Rad CFX Connect and Bio-Rad CFX96 Touch

How do I obtain TaqMan documentation files for my TaqMan array plates?

Beginning in April 2016, TaqMan documentation files for TaqMan Array Plates (assay information files (AIF), experimental setup files, and plate layout files) can be downloaded from the Thermo Fisher Scientific website at https://www.thermofisher.com/taqmanfiles. To obtain your TaqMan documentation files:
• Select the appropriate product type.
• Enter the information being requested for the product type.
• Fixed TaqMan array plates: Enter the Part number and Batch number
• TaqMan Flexible Content panel array plates/Custom TaqMan array plates: Enter the Sales Order number and Batch number
• Click Submit.

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