QuantStudio™ 12K Flex Real-Time PCR System, 96-well block, desktop
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QuantStudio™ 12K Flex Real-Time PCR System, 96-well block, desktop
Applied Biosystems™

QuantStudio™ 12K Flex Real-Time PCR System, 96-well block, desktop

The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied BiosystemsRead more
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44710871 system
Catalog number 4471087
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The QuantStudio™ 12K Flex system is a highly flexible, comprehensive real-time PCR platform that leverages the best of Applied Biosystems real-time technology in a single instrument. The QuantStudio™ 12K Flex Real-Time PCR System includes easy-to-change blocks that can accommodate OpenArray® plates, TaqMan® array cards, 384-well, 96-well, and FAST 96-well plates allowing you to select the format that is right for your project whether it’s now or in the future, for maximum flexibility and consistency in your research.

Thermo Fisher Cloud
Thermo Fisher Cloud provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the Thermo Fisher Cloud are web browser-based software tools that are flexible, fast and easy to use. These software tools are compatible with Mac or PC as well as most Applied Biosystems instrument file types.

The QuantStudio™ 12K Flex System’s intuitive software, easy touch screen setup, automation integration, effortless block exchange, and easy-to- use OpenArray® components are designed to let you get started right away and reduce hands on time. Plan for the future and save time, money, and space in your lab with the only all-in-one qPCR instrument enabling a broad range of applications with maximum throughput.

Flexibility & Scalability
Includes five interchangeable blocks: The QuantStudio™ 12K Flex blocks accomodate 96-well, FAST 96-well, 384-well, TaqMan® array cards, and OpenArray® plates, for effortless scaling from 1 to 12,000 data points in a single run. Increase throughput as you dive deeper into your research, using just one instrument. As you begin your project, start with 96-well format experiments and then scale up to a higher throughput to get the statistically significant answers you need. Additionally, you can easily validate and screen your targets of interest with a selected panel of genes using novel formats such as the TaqMan® array cards and OpenArray® plates.

• Compatible with a full range of TaqMan® assays:
The QuantStudio™ 12K Flex System is optimized to enable clear, clean data for all TaqMan® assays that might be used across a broad range of applications, including gene expression, microRNA and small noncoding RNAs, SNP genotyping, protein analysis, protein thermal shift, high resolution melt, copy number variation, and pathogen detection.

• Provides a seamless switch from qPCR to digital PCR:
Increase the precision and sensitivity of your nucleic acid experiment by switching from qPCR to digital PCR mode using QuantStudio™ Digital PCR kits and DigitalSuite™ Software with the QuantStudio™ OpenArray® block.

• Delivers peace of mind:
Leave early on a Friday and monitor runs from home, knowing that the system can safely store over 100 runs.

Maximum Throughput
Enhanced OptiFlex® System: Building upon Applied Biosystems’ proprietary technology, the Enhanced OptiFlex® System provides enhanced fluorescence detection for the accurate and sensitive data analysis necessary to collect 12,000 data points in a single run.

• Unprecedented coverage and sample throughput:
The OpenArray® format accelerates genomic validation and screening, driving over 150,000 data points per work day, enabling most projects to be completed in days instead of weeks.

• Simple OpenArray® workflow:
QuantStudio™ Open Array® plates arrive in a case to facilitate ease of handling and ensure optimal data quality upon loading of samples using the Accufill™ System. Easily load four arrays into the QuantStudio™ 12K Flex instrument to achieve over 12,000 data points. Integrated sample tracking software lets you easily track your samples throughout the course of your project.

• Integrated analysis and quality systems:
New intuitive touch-screen interface and integrated calendar feature allows for scheduling time of use on the instrument. Additional analysis software such as DigitalSuite™ v1.0, ExpressionSuite v1.0, and Genotyper v1.1 is available to help you analyze hundreds of targets and samples and achieve the statistical power you need.

QuantStudio™ 12K Flex Software
• Intuitive interface and innovative design for novice and experienced users
• Convenient walk-away operation
• Improved data analysis across block types
• Fully compatible with high-throughput environments

Expandable Software Architecture
Optional add-ons are available to upgrade the existing software:
21 CFR Part 11 compliance: This optional SAE module assists with Security, Auditing, and Electronic signature of records for full data traceability.

• High Resolution Melt (HRM):
Using the built-in MeltDoctor™ protocols and calibrations, you can spend less time optimizing the run and more time customizing your results. Define the number of variants you want to see by quickly changing analysis settings.

• DigitalSuite™ Software v1.0:
For OpenArray® block users needing sensitivity for rare targets and precision to measure small differences in target abundance.

• ExpressionSuite Software v1.0:
our latest stand-alone gene expression software with enhanced analysis tools that can analyze over 200 384-well plates or 10 OpenArray® plates.

Learn more about available financing options and programs ›

Specifications
Detection MethodSYBR, Primer-Probe Detection
Dimensions51 cm (W) x 74 cm (H) x 67 cm (D)
Display TypeTouchscreen
Dynamic RangeUp to 9 Logs of Linear Dynamic Range
Electrical Requirements60 Hz
External ComputerComputer and Monitor
For Use With (Equipment)QuantStudio™
Format96-well Plate
High-throughput CompatibilityHigh-throughput Compatible, Multiplexing
Optics6 Emission Filters (500-720 nm), 6 Excitation Filters (450-670 nm), 21 Color Options
Other Format Options96-well Fast Block Upgrade Option, OpenArray™ Block Upgrade Option, TaqMan™ Array Card Block Upgrade Option, 384-well Block Upgrade Option
Product LineQuantStudio™
ProgramsRemote Monitoring of up to 10 Instruments
Quantity1 system
Reaction SpeedStandard
Reaction Volume Range10–100 μL (96-well Plate)
SensitivityDown to 1 Copy
Voltage100 to 240 V
Weight69 kg (152 lb.)
Block FormatInterchangeable
Unit SizeEach
Contents & Storage
This instrument comes with an 96-well Block.
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Limited Use Label Licenses (LULL)

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Notice to Purchaser: The purchase of this instrument conveys to the purchaser the limited, non-transferable right to use the purchased instrument only, under intellectual property rights that are owned and/or controlled by Life Technologies and relate specifically to the instrument. Purchase of the instrument includes the right to use the instrument for internal research and to perform services (including the right to report the results of services for a fee) by the purchaser only, but does not convey rights to use any other products, reagents, assays or methods such as the 5’ nuclease assay process. The sale of this instrument is expressly conditioned on the purchaser not reselling, repackaging, or distributing this instrument, or any of its components, and no such rights are conveyed expressly, by implication, or by estoppel. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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Frequently asked questions (FAQs)

Please follow the instructions listed in this manual (https://licensing.appliedbiosystems.com/web/static/tools/manual.pdf).

For problems with license renewal, please send an email to instrumenthardwaresupport@thermofisher.com (for North America customers) or eurotech@thermofisher.com (for EMEA customers), with the computer ID and license key (AID-number) so you can be assisted.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

When this happens, the analysis was set to use ‘Relative Threshold' but the CRT values do not show up in the exported data. (Note: To use ‘Relative Threshold', go to “Analysis Settings”, and under the default Ct Settings tab, choose ‘Relative Threshold' under “Algorithm Settings”.)

To export the data, go to ‘Export' and make sure the ‘Results' section is checked. You will notice that the column header will still say ‘Ct' even if you have CRT in the well table like above. If you export the data but notice that the numbers still correspond to the ‘Ct' values (and not the CRT values), then you will need to use the ‘Load Export Set' button. Just click ‘OK' to the pop-up window. and the changes will be applied. The next time you export, the values should reflect the CRT values.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Sometimes an error can occur when the file is downloaded to the computer. When this occurs you may see an abrupt break in the data. If you see this, you can repair the data by going to Analysis then Analyze from Raw Data. You should then see a fixed plot. Please note that you would need to have at least v1.2 of the QuantStudio 12K Flex software to use this feature. If you do not have this version, or if you do but the error persists, please send your data file to techsupport@thermofisher.com so that we can attempt to recover the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

The ‘reveal traces' feature for genotyping runs allows you to trace the clusters throughout the PCR process. For some assays you may find better cluster discrimination at, say, cycle 40, as opposed to cycle 50. To use this feature, the amplification data needs to have been collected for the run.

- Go to 'Analysis Settings'.
- Under the default ‘Call Settings' tab, Choose 'Analyze Real-Time Run (?) Data'.
- Click ‘Apply Analysis Settings'.
- Under the Allelic Discrimination Plot, check the box next to ‘Reveal Traces'. You can then move the slide bar to see how the data change with the cycle number.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Please follow the directions in this user guide (https://licensing.appliedbiosystems.com/web/static/tools/manual.pdf) to manage your license (including transfer and renewing a license).

If you are unable to resolve the issue after following these steps, please contact us at instrumentservices@thermofisher.com for further assistance.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

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