Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Quantity |
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4471087 | 1 system |
Notice to Purchaser: The purchase of this instrument conveys to the purchaser the limited, non-transferable right to use the purchased instrument only, under intellectual property rights that are owned and/or controlled by Life Technologies and relate specifically to the instrument. Purchase of the instrument includes the right to use the instrument for internal research and to perform services (including the right to report the results of services for a fee) by the purchaser only, but does not convey rights to use any other products, reagents, assays or methods such as the 5’ nuclease assay process. The sale of this instrument is expressly conditioned on the purchaser not reselling, repackaging, or distributing this instrument, or any of its components, and no such rights are conveyed expressly, by implication, or by estoppel. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'Please follow the instructions listed in this manual (https://licensing.appliedbiosystems.com/web/static/tools/manual.pdf).
For problems with license renewal, please send an email to instrumenthardwaresupport@thermofisher.com (for North America customers) or eurotech@thermofisher.com (for EMEA customers), with the computer ID and license key (AID-number) so you can be assisted.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
When this happens, the analysis was set to use ‘Relative Threshold' but the CRT values do not show up in the exported data. (Note: To use ‘Relative Threshold', go to “Analysis Settings”, and under the default Ct Settings tab, choose ‘Relative Threshold' under “Algorithm Settings”.)
To export the data, go to ‘Export' and make sure the ‘Results' section is checked. You will notice that the column header will still say ‘Ct' even if you have CRT in the well table like above. If you export the data but notice that the numbers still correspond to the ‘Ct' values (and not the CRT values), then you will need to use the ‘Load Export Set' button. Just click ‘OK' to the pop-up window. and the changes will be applied. The next time you export, the values should reflect the CRT values.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Sometimes an error can occur when the file is downloaded to the computer. When this occurs you may see an abrupt break in the data. If you see this, you can repair the data by going to Analysis then Analyze from Raw Data. You should then see a fixed plot. Please note that you would need to have at least v1.2 of the QuantStudio 12K Flex software to use this feature. If you do not have this version, or if you do but the error persists, please send your data file to techsupport@thermofisher.com so that we can attempt to recover the data.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
The ‘reveal traces' feature for genotyping runs allows you to trace the clusters throughout the PCR process. For some assays you may find better cluster discrimination at, say, cycle 40, as opposed to cycle 50. To use this feature, the amplification data needs to have been collected for the run.
- Go to 'Analysis Settings'.
- Under the default ‘Call Settings' tab, Choose 'Analyze Real-Time Run (?) Data'.
- Click ‘Apply Analysis Settings'.
- Under the Allelic Discrimination Plot, check the box next to ‘Reveal Traces'. You can then move the slide bar to see how the data change with the cycle number.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Please follow the directions in this user guide (https://licensing.appliedbiosystems.com/web/static/tools/manual.pdf) to manage your license (including transfer and renewing a license).
If you are unable to resolve the issue after following these steps, please contact us at instrumentservices@thermofisher.com for further assistance.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
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