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Ion Plus Fragment Library Kit
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Ion Plus Fragment Library Kit
Ion Torrent™

Ion Plus Fragment Library Kit

As an integral component of the Personal Genome Machine™ sequencing platform, the Ion Plus Fragment Library Kit is designed toRead more
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Catalog NumberQuantity
447125210 Reactions
Catalog number 4471252
Price (USD)
952.00
Each
Add to cart
Quantity:
10 Reactions
Price (USD)
952.00
Each
Add to cart

As an integral component of the Personal Genome Machine™ sequencing platform, the Ion Plus Fragment Library Kit is designed to produce high quality DNA libraries via multiple possible workflows. The economically priced Ion Plus Fragment Library Kit provides low cost sample preparation, bringing next generation sequencing to every lab. This revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries when combined with either physical shearing methods or our proprietary Ion Shear™ enzymatic fragmentation technology (Ion Xpress Plus Library Kit, Cat. No. 4471269), thus saving time and cost. With significantly higher yields than other library construction techniques, high quality libraries can be attained with as little as 100 ng input DNA. In addition, this highly efficient library construction process enables the creation of 'amplification-free' libraries from <1 μg input material. This kit is also extremely flexible in that it is compatible with multiple physical shearing and library size selection methods.

The Ion Plus Fragment Library Kit contains sample preparation reagents for library construction of up to 20 DNA libraries (depending on input DNA type and amount) for semiconductor sequencing. Store components at -20°C.

The Ion Plus Fragment Library Kit offers you:

  • Lower input requirement with as little as 100 ng starting material
  • Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes with a choice of mix-and-match workflows
  • Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

A Single-Day Workflow: The Next Stage in a Sequencing Revolution

With a quick turnaround time the Ion Plus Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your samples possible in less time than ever before. Furthermore, the Ion Plus Fragment Library Kit is compatible with a variety of DNA shearing and library size-selection methodologies allowing you to tailor your workflow to suit your project needs.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Post-Light™ Ion Semiconductor Sequencing
For Use With (Equipment)Ion GeneStudio™ S5 Series Sequencer, Ion Chef™ System
High-throughput CompatibilityHigh-throughput Compatible, Multiplexing
LibrariesFragment Library
Product LineIon Torrent™, Ion Xpress™
Quantity10 Reactions
Sample TypeDNA
Sequencing TypeGenome & DNA Sequencing
Shipping ConditionDry Ice
Workflow StepLibrary Generation
Unit SizeEach
Contents & Storage
• 5 x End Repair Buffer, 1 tube, 400 μL, store at -10°C to -30°C
• End Repair Enzyme, 1 tube, 20 μL, -10°C to -30°C
• 10 x Ligase Buffer, 1 tube, 200 μL, -10°C to -30°C
• DNA Ligase, 1 tube, 40 μL, -10°C to -30°C
• Adapters, 1 tube, 100 μL, -10°C to -30°C
• Platinum™ PCR SuperMix High Fidelity, 2 tubes, 1 mL, -10°C to -30°C
• Library Amplification Primer Mix, 1 tube, 100 μL, -10°C to-30°C
• Nick Repair Polymerase, 1 tube, 160 μL, -10°C to -30°C
• dNTP Mix, 1 tube, 40 μL, -10°C to -30°C
• Low TE, 2 tubes, 1.5 mL, 15°C or -10°C to -30°C

Frequently asked questions (FAQs)

What are the recommended conditions for long-term storage of my libraries?

For long-term storage, libraries should be stored concentrated (dilutions recommended for template preparation are not intended for long term storage) in LoBind tubes in low TE in non-frost free freezer at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

If adapter dimers are present in my library, can they be detected using the Ion Library TaqMan Quantitation Kit (qPCR)?

No, the Ion Library TaqMan Quantitation Kit for qPCR library quantification is unable to differentiate amplifiable primer-dimers from library fragments. We recommend assessing the library size distribution, including checking for the presence of adapter dimers, using the Bioanalyzer instrument. Libraries containing adapter dimers will have sharp peaks at ~70 bp for non-barcoded libraries or ~90 bp for barcoded libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I tried quantifying my amplified library with both the Ion Library Quantitation Kit (qPCR) and the Bioanalyzer instrument. The concentration according to qPCR is lower than the Bioanalyzer instrument. Which concentration should I use?

We recommend using the qPCR result, as quantification by qPCR is generally more accurate than quantification using the Bioanalyzer instrument.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Ion Library Equalizer Kit (Cat. No. 4482298) be used to normalize 400-base read libraries?

Currently, the Ion Library Equalizer Kit is validated and only recommended for use with up to 300-base read libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

For quantification of my library on the Bioanalyzer instrument, do I have to amplify my library first?

We highly recommend quantifying unamplified libraries using qPCR, as it specifically measures the amplifiable (or usable) library fragments present. Unamplified libraries will contain unadapted and improperly adapted fragments, which will be included in quantification data obtained if using the Bioanalyzer instrument. Library quantification on the Bioanalyzer instrument using the Agilent High Sensitivity DNA Kit is only recommended for amplified libraries, as the library amplification process selects for properly adapted library fragments.

The Bioanalyzer instrument can be used to assess the size distribution of both amplified and unamplified libraries. Please see the Ion Xpress Plus gDNA Fragment Library Preparation User Guide (Pub. No. 4471989) for detailed information. If using the Bioanalyzer instrument for quantification of unamplified libraries, please note that this method will generally underestimate the concentration of usable library fragments and a library input titration may be necessary to optimize template preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

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