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Thermo Scientific™
POROS™ GoPure™ XS Pre-packed Column, 0.5 x 5 cm, 1 mL
Thermo Scientific™ POROS™ GoPure™ 0.5 cm pre-packed columns are intended for the screening of POROS ion exchange resins or for small scale purification.
| Katalognummer | Menge |
|---|---|
| 4481317 | 1 Column |
Katalognummer 4481317
Preis (EUR)
141,00
Each
-
Menge:
1 Column
Preis (EUR)
141,00
Each
POROS GoPure 0.5 cm pre-packed columns are intended for the screening of POROS ion exchange resins or for small scale purification.
These pre-packed columns are ready to connect and have the following features:
- Dimensions of 0.5 x 5 cm, with a nominal bed volume of 1 mL, providing a uniform screening platform
- Direct connection to standard chromatography systems
- Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates
The resin in this POROS GoPure XS Column has a sulphopropyl functional group for strong cation exchange chromatography.
- Convenient—ready-to-run columns simplify and speed POROS resin evaluations
- Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
- Simplified scalability—the consistency and range of the POROS GoPure family of pre-packed columns simplifies process scale-up and process modeling
POROS GoPure 0.5 cm pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended ≥3 min residence time (0.3 mL/min) on this 5 cm length column equates to 400 cm/hr on a 20 cm bed height. Detailed specifications may be found in the POROS GoPure 0.5 cmD Operating Instructions below.
Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography™ resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Zur Verwendung mit (Geräte)FPLC
Hochdurchsatz-KompatibilitätAutomatisierte Protokolle
Länge (metrisch)5 cm
Max. Betriebsdruck30 bar
Partikelgröße50 μm
ProduktlinieGoPure™
Menge1 Column
ProbentypBiopharmaceutical Purification Sample, Cell Culture Supernatants, Serum
SäulentypCation Exchange
Diameter (Metric) Inner Column0.5 cm
Flussrate0.33 mL/min.
Stationäre PhaseSulphopropyl
Volumen (metrisch)1 mL
Unit SizeEach
Inhalt und Lagerung
Shipped in 20% ethanol at 2°C to 30°C
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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product, under intellectual property rights that are owned and/or controlled by Life Technologies and relate specifically to the product, to perform (a) internal research for the sole benefit of the purchaser; (b) quality control testing conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties; and (c) performance of research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. The purchase of this product does not grant the purchaser any additional rights, including, without limitation, the right to transfer or resell the product in any form, the right to use the product as a therapeutic agent or diagnostics test component, or to use the product to perform tests other than what is indicated in this Limited Use Label License on a contract or fee per test basis for or on behalf of third parties. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'Häufig gestellte Fragen (FAQ)
All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
UPLC is typically described as chromatography with sub-2 µm particles, which drives higher efficiency/better separation independent of operating flow rate. As particle size decreases, backpressure increases so the UPLC systems tend to have higher pressure ratings compared to HPLC systems. The flow rates on UPLC systems would be sufficient for POROS columns. Pump capabilities are typically 0.1-1.0 mL/min, so not as fast as a HPLC, but sufficient. Hold-up volumes on UPLC systems are lower than HPLCs, so this can help with sensitivity and efficiency. So from a pressure and flow standpoint there should be no challenge to operate a POROS CaptureSelect column on a UPLC system.
That being said, care should be taken running POROS columns on UPLC systems. Higher pressure safety interlock MUST be set at the rated column pressure, typically 180 bar. HPLC column hardware is not rated to operate at the static pressures possible and typical on UPLC systems. A clog or the like on a UPLC system could generate unsafe pressures on any HPLC-rated column before the default UPLC safety pressure interlock is tripped.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
POROS resins rehydrate quite well. Here is the procedure that we recommend:
1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.