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NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
Thermo Scientific™

NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer

Thermo Scientific NHS-Rhodamine is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins andRead more
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4640625 mg
Catalog number 46406
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194.00
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25 mg
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Thermo Scientific NHS-Rhodamine is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Features of NHS-Rhodamine :

Amine-specific labeling—NHS-ester varieties of rhodamine efficiently label antibodies and other purified proteins at primary amines (lysine side chains)
Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence

NHS Rhodamine is an amine-reactive derivative of rhodamine dye that has wide-ranging application as antibody and other probe labels for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as western blotting and ELISA.

Applications:
• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on western blots

Properties of Rhodamine Dyes:
Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.

NHS-Rhodamine is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to TRITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling.

Application Data:

Related Products
Pierce™ NHS-Rhodamine Antibody Labeling Kit
TRITC (5/6-tetramethyl-rhodamine isothiocyanate), mixed isomer
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
DescriptionPierce NHS-Rhodamine
Emission575
Excitation552
Label or DyeRhodamine Isomers, TMR (Tetramethylrhodamine), TRITC Isomers
Product TypeNHS-Rhodamine
Quantity25 mg
Reactive MoietyActive Ester, Succinimidyl Ester, NHS Ester
SolubilityDMF (Dimethylformamide)
Label TypeClassic Dyes
Product LinePierce™
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
AE4671641Certificate of AnalysisMay 01, 202546406
ZL4570331Certificate of AnalysisFeb 12, 2025DNU-46406, 46406
ZI4451607Certificate of AnalysisOct 15, 202446406
ZE4287491Certificate of AnalysisMay 29, 202446406
YK4114511Certificate of AnalysisJan 25, 202446406
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Safety Data Sheets

Frequently asked questions (FAQs)

Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).

Dye Excitation Emission EC
Rhodamine B 568 583 88,000
Rhodamine 123 507 529 101,000
Rhodamine 110 499 521 92,000
Rhodamine 6G 528 551 105,000
XRITC 572 596 92,000


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