The Applied Biosystems Axiom Bovine Genotyping v3 Array has >63,000 markers and is designed for genomic and trait selection and parentage verification of cattle. The single-nucleotide polymorphisms (SNPs) on the array were selected based on their position in the bovine genome, ensuring even spacing. These include VIP trait-associated SNPs that are publically available, ISAG core parentage markers, and 44,000 markers from the Council on Dairy Cattle Breeding (CDCB). SNPs optimized for STR imputation (1) are also included in the array content. This array is also available in mini-96 format.

Traits associated with fertility are on the Y-chromosome, so over 500 markers on the Y-chromosome are included in this array. The array also contains >10,000 Bos indicus markers of approximate equal coverage across the Bos indicus genome to avoid gaps.

Applications
• Parentage analysis
• Identification of economically important traits
• Dairy evaluation
• Genetic improvement of pure lines

Required products
Axiom Long Format Export Tool
Command Console Software
GeneTitan Multi-Channel Instrument
Axiom Analysis Suite Software

Reference
1. McClure MC, Sonstegard TS, Wiggans GR, et al. Imputation of microsatellite alleles from dense SNP genotypes for parentage verification across multiple Bos taurus and Bos indicus breeds. Front Genet. 2013;4:176.