Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Capacity (Metric) |
---|---|
5943072005 | 5 mL |
5943072001 | 1 mL |
CaptureSelect C-tagXL MiniChrom™ columns are designed for bench-scale resin screening, process development, and sample preparation.
These pre-packed columns are ready to connect and have the following features:
The 1-mL columns can be used for quick screening of application feasibility and lab-scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5-mL column allows initial process development on a bench scale.
CaptureSelect C-tagXL Affinity Matrix has a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine). CaptureSelect C-tagXL Affinity Matrix purifies the C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high-activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.
Features of this affinity matrix include:
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics: