Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Proteinase K Kits. A significant advance in sample preparation for biopharmaceutical research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.
Catalog Number
60109-107
Unit Size
Each
Description
SMART Digest Proteinase K Kit, with Collection plate
Price (USD)
966.17
Full specifications
Quantity
Description
SMART Digest Proteinase K Kit, with Collection plate
Capture
None
Cleavage
Peptide bond adjacent to carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups
Enzyme
Proteinase K
Format
SMART Digest Resin Pre-filled into PCR Tubes
For Use With (Application)
Peptide Quantitation, Peptide Mapping
Product Line
SMART Digest
Type
Protein Digestion
Includes
SMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate
SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses proteinase K, which cleaves the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups.
Easy and Simple
Significantly increased speed of sample processing
Simple process
Easily automate entire protocol for high-throughput processing
Reliable Results
Increased reproducibility over existing protocols
Higher sensitivity
Higher data confidence
Significantly Reduces Sample Preparation Time
Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.
Cleaner Samples
Reduction in the number of chemicals used and the immobilized proteinase K design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. The results are easier to interpret.
Couple with SOLAμ SPE Plates for Desalting
When used with the SMART Digest for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.