BOVIGAM™ 2G TB Kit
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BOVIGAM™ 2G TB Kit
Thermo Scientific™

BOVIGAM™ 2G TB Kit

The BOVIGAM® 2G kit is a blood-based assay of cell-mediated immunity that has been validated together with tuberculin PPDs andRead more
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Catalog number 63330
Price (USD)/ 300 samples
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The BOVIGAM® 2G kit is a blood-based assay of cell-mediated immunity that has been validated together with tuberculin PPDs and a panel of stimulation antigens. The combined use of these different stimulation antigens with tuberculin PPDs allows adjustment of the application of the test to specific TB situations. It is based on the detection of cell-mediated immune response to infection with Mycobacterium bovis in cattle, sheep, goats, buffalo, bison, and other bovidae. The BOVIGAM® 2G test involves two stages. In the first stage, blood samples are incubated overnight with antigens, such as tuberculin purified protein derivative (PPD) or the newly developed 'peptide cocktails', to stimulate lymphocytes to produce IFN-γ. In the second stage, IFN-γ in the plasma supernatants of each blood aliquot is determined using a sandwich ELISA. IFN-γ present in the sample binds to anti-bovine IFN-γ monoclonal antibodies on a solid support and is visualized with a second anti-IFN-γ antibody labeled with an enzyme that generates a color signal. Color development is proportional to the amount of bound IFN-γ.
Specifications
Assay SensitivitySee product documentation
Immunoassay Kit FormatOther Format
Product LineBOVIGAM™, Prionics™
Protein FamilyOther Proteins
Target SpeciesBovine
Gene ID (Entrez)Non-Gene
Gene SymbolNon-Gene
Quantity300 samples
Target Kit Named2G TB
Target Organism ClassMycobacterium bovis
Target Species ValidatedBovine, Goat, Sheep
Unit Size300 samples
Contents & Storage
10 plates