PBS (10X), pH 7.4
Save up to 29% on your order of cell culture products. View promotion details >
PBS (10X), pH 7.4
Gibco™

PBS (10X), pH 7.4

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such asRead more
Promo ImagePromotion
Promo code:RCELL25
Save up to 29% across the cell culture workflow - research begins with cells
From now until Dec 31, get up to 29% off media, sera, buffers, reagents and other cell culture products to advance your researchLearn More
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
70011069Promo Image10 x 500 mL
70011044Promo Image500 mL
Catalog number 70011069
Price (USD)
584.00
Each
Add to cart
Quantity:
10 x 500 mL
Price (USD)
584.00
Each
Add to cart

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution (see protocol for details).

We offer a variety of Gibco™ PBS formulations for a range of cell culture applications.


This PBS is manufactured as follows:
Without
• Calcium
• Magnesium
• Phenol Red


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
ColorWhite
Dilution10 X
For Use With (Application)Mammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality2800 - 3100 mOsm/kg
Phenol Red IndicatorNo Phenol Red
Product LineGibco™
Quantity10 x 500 mL
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Product TypePhosphate Buffered Saline (PBS)
Solution TypePhosphate Buffered Saline
pH7.4
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Media Formulations

Frequently asked questions (FAQs)

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is a precipitate in PBS normal?

No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between PBS and DPBS?

PBS has no potassium chloride (KCl) and it has a slightly lower level of phosphate (PO4) than DPBS. PBS is used for washing cells when viability isn't critical.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link