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Application
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Phospho-Paxillin (Ser178) Antibody (711418) in ICC/IF
For immunofluorescence analysis, MDA-MB-231 cells were fixed and permeabilized for detection of endogenous Paxillin pS178 using Anti- Paxillin pS178 Recombinant Rabbit Superclonal™ Antibody (Product # 711418, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were staine... View More
Product Details
711418
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
21HCLC
Immunogen
Peptide corresponding to Human PXN (aa 173-181)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633015
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Rat and Dog
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ16691; hCG_1778014; PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134
Gene Aliases: AW108311; AW123232; Pax; PXN
UniProt ID: (Human) P49023, (Mouse) Q8VI36
Entrez Gene ID: (Human) 5829, (Mouse) 19303
muscle contraction
cell adhesion
cell-matrix adhesion
signal transduction
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
cellular response to reactive oxygen species
vascular endothelial growth factor receptor signaling pathway
growth hormone receptor signaling pathway
activation of MAPK activity
movement of cell or subcellular component
cytoskeleton organization
integrin-mediated signaling pathway
regulation of cell shape
peptidyl-tyrosine phosphorylation
lamellipodium assembly
substrate adhesion-dependent cell spreading
positive regulation of protein kinase activity
focal adhesion assembly
branching morphogenesis of an epithelial tube
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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