Uracil-DNA Glycosylase, heat-labile
Uracil-DNA Glycosylase, heat-labile
Thermo Scientific™

Uracil-DNA Glycosylase, heat-labile

Excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone.
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Catalog NumberQuantity
78310100UN100 U
Catalog number 78310100UN
Price (USD)
168.65
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183.00
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Quantity:
100 U
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Price (USD)
168.65
Online Exclusive
183.00
Save 14.35 (8%)
Each
Add to cart

Uracil-DNA Glycosylase excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone. This cleavage generates alkali sensitive apyrimidinic sites that are blocked from replication by DNA polymerase or prevented from becoming a hybridization site.

Double and single-stranded dU-containing DNA are substrates for Uracil-DNA Glycosylase whereas RNA and normal dT-containing DNA are not.

Uracil-DNA Glycosylase can also be used to increase the cloning efficiency of PCR products having dU-containing primers incorporated into them as well as increasing the efficiency of site-directed mutagenesis.

The Uracil-DNA Glycosylase derived from Gadus morhua has all the attributes of the enzyme derived from E. coli with the added benefit of being heat-labile. It is completely and irreversibly inactivated after 10 minute incubation at 50°C.

Purity:

The enzyme is chromatographically purified and tested for contaminating endonucleases and exonucleases.

Storage Buffer:

50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.

Assay Conditions:

The reaction mixture contains 70 mM Tris-HCl, pH 8.0, 10 mM NaCl, 1 mM EDTA, 100 μg/mL BSA, 3H-dUTP labeled DNA, and Uracil-DNA Glycosylase. Incubation is at 37°C for 1 hour.

Unit Definition:

One unit is the amount of enzyme required to liberate 1 nmol uracil from dU-containing DNA in one hour at 37°C.

Concentration:

1 unit/μL

Applications:

  1. Study of DNA repair and mutation detection.
  2. Increase cloning efficiency of PCR products.
  3. Increase the efficiency of site-directed mutagenesis.
  4. Study of protein-DNA interactions.

Source:

Recombinant from Gadus morhua (cod liver)

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible BufferStorage Buffer
Product TypeUracil DNA Glycosylase
Quantity100 U
EnzymeGlycosylase
Unit SizeEach
Contents & Storage
Shipped on dry ice. Store at -20°C.
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Frequently asked questions (FAQs)

What is UDG?

UDG (uracil-DNA-glycosylase) refers to a superfamily of enzymes comprising six sub-families. Family I UDG enzymes are called UNG, after the uracil-N-glycosylase gene. The terms UDG and UNG are commonly used interchangeably because they perform the same function in qPCR, namely, to remove Uracil from dU-containing DNA to prevent carryover contamination from previous PCRs. See the following link: https://www.thermofisher.com/uk/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/real-time-pcr-basics/what-is-ung-udg.html

How many samples from the Oncomine Comprehensive Assay Plus, manual library preparation can I run on the Ion 550 Chip?

We recommend sequencing up to 5 DNA or RNA samples (4 samples and a non-template control (NTC)) on a single Ion 550 Chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Oncomine Comprehensive Assay Plus, manual library preparation?

The Oncomine Comprehensive Assay Plus, manual library preparation (Cat. No. A46721) is designed to prepare barcoded sample libraries from DNA and RNA. The kit consists of Oncomine Comprehensive Assay Plus DNA (2-pool) (Part No. A45615), RNA Oncomine Comprehensive Assay v3M (2-pool) (Part No. A33637), and two kits of Ion AmpliSeq Library Kit Plus (Cat. No. 4488990). Sufficient reagents are provided to prepare libraries from 24 samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How much of each sample is needed for the Oncomine Comprehensive Assay Plus, manual library preparation?

10 ng of nucleic acid input per pool (20 ng of DNA and 20 ng of RNA) isolated from FFPE samples, including fine needle biopsies, is needed for the Oncomine Comprehensive Assay Plus, manual library preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What types of sample is the Oncomine Comprehensive Assay Plus, manual library preparation compatible with?

The assay is optimized for use with formalin-fixed paraffin embedded (FFPE) samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

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