Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sampleRead more
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Catalog NumberQuantity
784401 mL
7844224 x 100 μL
7844610 mL
784445 x 1 mL
Catalog number 78440
Price (USD)
300.65
Online Exclusive
335.00
Save 34.35 (10%)
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Quantity:
1 mL
Request bulk or custom format
Price (USD)
300.65
Online Exclusive
335.00
Save 34.35 (10%)
Each
Add to cart
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates.

Features of Halt Protease and Phosphatase Inhibitor Cocktail (100X):

Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers

Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube.

Related Products
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferLysis Buffer
InhibitorsProtease Inhibitor, Phosphatase Inhibitor
Quantity1 mL
Reagent TypeProtease Inhibitor, Phosphatase Inhibitor
FormLiquid
Product LineHalt™
Product TypeProtease and Phosphatase Inhibitor Cocktail
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C

Frequently asked questions (FAQs)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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