This PrimeFlow™ RNA Assay is an in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using fluorochrome-conjugated antibodies. Branched-DNA technology uses pairs of target-specific probes to amplify the detection of a RNA transcript, achieving between 8,000- to 16,000-fold signal amplification from a desired RNA transcript. PrimeFlow RNA Assay can detect mRNA, long non-coding RNA, viral RNA, and telomere DNA. The detection of microRNA is also possible and is optimal when using the PrimeFlow™ microRNA Pretreatment Buffer (cat. 88-18006) and protocol. PrimeFlow RNA Assay is compatible with human and mouse cells. Other mammalian and non-mammalian cells may be compatible

This PrimeFlow RNA Assay contains all the reagents needed to conduct the assay. Positive control probes and target probes for genes of interest are sold separately.

Reported Application
RNA Flow Cytometry