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HisPur™ Ni-NTA Spin Columns, 0.2 mL
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Thermo Scientific™

HisPur™ Ni-NTA Spin Columns, 0.2 mL

Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.FeaturesRead more
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Catalog NumberQuantity
8822425 Columns
Catalog number 88224
Price (USD)
250.65
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282.00
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25 Columns
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Price (USD)
250.65
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282.00
Save 31.35 (11%)
Each
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Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features of HisPur Ni-NTA Resin:

High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
Versatile—purify proteins using native or denaturing conditions
Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Cost-effective—reuse the same batch of resin at least five times
Easy to use—pre-formulated buffers available for kit formats
Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags, the most popular being the polyhistidine (6xHis) tag. The method used to purify recombinant His-tagged proteins is immobilized metal affinity chromatography (IMAC), consisting of chelating resins charged with either nickel or cobalt ions that coordinate with the histidine side chains.

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.

HisPur Ni-NTA Resin and HisPur Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater purity but usually less yield in His-tagged protein purification.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Ni-NTA Resin
HisPur™ Ni-NTA Spin Purification Kit, 0.2 mL
HisPur™ Ni-NTA Spin Plates
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeAffinity, Agarose Resin
DescriptionHisPur Ni-NTA Spin Columns, 0.2mL
FormatMicrocentrifuge, Spin Column
Quantity25 Columns
Stationary PhaseDivalent Nickel, Metal Chelate
TargetHis-Tagged Protein
Capacity (Metric)0.2 mL
FormLiquid Suspension
Product LineHisPur™
TypeSpin Column
Unit SizeEach
Contents & Storage
Store at 4°C
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Frequently asked questions (FAQs)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between HisPur Nickel Resin and HisPur Cobalt Resin?

Nickel has a higher affinity for histidines than does cobalt. It binds multiple histidines more tightly than cobalt, and requires more stringent conditions than is necessary for cobalt. The lower affinity of cobalt for multiple histidines typically results in less nonspecific binding of histidine-rich proteins that lack a his-tag compared to nickel resins.

HisPur Ni-NTA resin has a high capacity of up to 60 mg of 6xHis-tagged protein per milliliter. It is a versatile resin that can work under both native and denaturing conditions, and can also be used with a variety of lysis reagents and buffer additives.

HisPur Cobalt resin utilizes proprietary tetradentate chelating resin charged with cobalt. This system recovers highly purified protein with lower imidazole concentrations and has low metal leeching properties.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
3212156ACertificate of AnalysisJun 02, 202588224
3212156Certificate of AnalysisJun 02, 202588224
AA402943Certificate of AnalysisFeb 20, 202588224
SPECSCertificate of AnalysisOct 15, 202488224
ZG395683Certificate of AnalysisAug 17, 202488224
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