Mitochondria Isolation Kit for Tissue
Mitochondria Isolation Kit for Tissue
Thermo Scientific™

Mitochondria Isolation Kit for Tissue

The Thermo Scientific Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples inRead more
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Catalog NumberQuantity
89801115 mL
Catalog number 89801
Price (USD)
365.65
Online Exclusive
392.00
Save 26.35 (7%)
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Quantity:
115 mL
Request bulk or custom format
Price (USD)
365.65
Online Exclusive
392.00
Save 26.35 (7%)
Each
Add to cart
The Thermo Scientific Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour using reagent-based or Dounce homogenization methods.

Features of the Mitochondria Isolation Kit for Tissue:

Quick and Convenient—isolate intact mitochondria in less than 60 minutes
Versatile—offers two methods of isolation from both soft and hard tissue samples
Multi-sample format—reagent-based approach enables simultaneous processing of multiple samples
Optional alternate method—reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes
Bench-top compatible—both procedures can be performed using a microcentrifuge tube

This kit provides for two methods for mitochondria isolation. The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy.

Related Products
Mitochondria Isolation Kit for Cultured Cells
Lysosome Enrichment Kit for Tissues and Cultured Cells
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity115 mL
Reagent TypeOrganelle Isolation, Subcellular Fractionation
Sufficient For50 Isolations
FormLiquid
Product TypeCell Isolation Kit
Unit SizeEach
Contents & Storage
Upon receipt store kit at 4°C. Product is shipped at ambient temperature.

Frequently asked questions (FAQs)

Can you provide the shelf-life for the Mitochondria Isolation Kit for Tissues?

The Mitochondria Isolation Kit for Tissues is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What kits do you offer for cell fractionation?

We offer the following kits for cell fractionation:

Mem-PER Plus Membrane Protein Extraction Kit, Cat. No. 89842
NE-PER Nuclear and Cytoplasmic Extraction Reagents, Cat. No. 78835
Subcellular Protein Fractionation Kit for Cultured Cells, Cat. No. 78840
Subcellular Protein Fractionation Kit for Tissues, Cat. No. 87790
Mitochondrial Isolation Kit for Cultured Cells, Cat. No. 89874
Mitochondrial Isolation kit for Tissue, Cat. No. 89801
Lysosome Enrichment Kit for Tissues and Cultured Cells, Cat. No. 89839

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Why is cell fractionation important?

Isolation of subcellular fractions and concentration of low-abundantce proteins allows for more efficient identification and study of the proteins of interest.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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