The Thermo Scientific Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples inRead more
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Catalog Number
Quantity
89801
115 mL
Catalog number 89801
Price (USD)
365.65
Online Exclusive
392.00
Save 26.35 (7%)
Each
Add to cart
Quantity:
115 mL
Request bulk or custom format
Price (USD)
365.65
Online Exclusive
392.00
Save 26.35 (7%)
Each
Add to cart
The Thermo Scientific Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour using reagent-based or Dounce homogenization methods.
Features of the Mitochondria Isolation Kit for Tissue:
• Quick and Convenient—isolate intact mitochondria in less than 60 minutes • Versatile—offers two methods of isolation from both soft and hard tissue samples • Multi-sample format—reagent-based approach enables simultaneous processing of multiple samples • Optional alternate method—reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes • Bench-top compatible—both procedures can be performed using a microcentrifuge tube
This kit provides for two methods for mitochondria isolation. The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy.
Upon receipt store kit at 4°C. Product is shipped at ambient temperature.
Frequently asked questions (FAQs)
Can you provide the shelf-life for the Mitochondria Isolation Kit for Tissues?
The Mitochondria Isolation Kit for Tissues is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Isolation of subcellular fractions and concentration of low-abundantce proteins allows for more efficient identification and study of the proteins of interest.