Chemiluminescent Nucleic Acid Detection Module Kit
Chemiluminescent Nucleic Acid Detection Module Kit
Thermo Scientific™

Chemiluminescent Nucleic Acid Detection Module Kit

The Thermo Scientific Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probesRead more
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Catalog NumberQuantity
8988010 Blots
Catalog number 89880
Price (USD)
594.65
Online Exclusive
676.00
Save 81.35 (12%)
Each
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Quantity:
10 Blots
Request bulk or custom format
Price (USD)
594.65
Online Exclusive
676.00
Save 81.35 (12%)
Each
Add to cart
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The Thermo Scientific Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications.

Nearly any DNA or RNA method based on nitrocellulose or nylon membranes and biotinylated probes can be detected with sensitivity equivalent to traditional radioactive (32P) systems. The module consists of the essential detection components used in the Thermo Scientific North2South Kit and Thermo Scientific LightShift EMSA Kit. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that provide for fast, trouble-free detection on film or by CCD camera. Obtain publication-quality blots having excellent signal and low background.

Features of the Chemiluminescent Nucleic Acid Detection Module:

Fast and sensitive—achieve the same speed and detection levels you've come to expect with Thermo Scientific North2South and LightShift Kits
Versatile—kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
Flexible—kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

Related Products
Nucleic Acid Detection Blocking Buffer
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LinePierce™
Product TypeNucleic Acid Detection Module Kit
Quantity10 Blots
Detection MethodChemiluminescence
FormatKit
Unit SizeEach
Contents & Storage
Sufficient For: 800 cm2 of membrane (10 mini-gel blots)
• Stabilized Streptavidin-HRP, 1.5 mL
• Luminol/Enhancer Solution, 80 mL
• Stable Peroxide Solution, 80 mL
• Blocking Buffer (Part No. 89880A), 500 mL
• Wash Buffer (4X), 500 mL
• Substrate Equilibration Buffer, 500 mL

Store at 4°C.
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Frequently asked questions (FAQs)

Will SuperSignal West Pico PLUS Chemiluminescent Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Pico PLUS Chemiluminescent Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Femto Maximum Sensitivity Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Femto Maximum Sensitivity Substrate was optimized for use in Western blot sand is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Dura Extended Duration Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Dura Extended Duration Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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