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GeneChip™ Hybridization, Wash, and Stain Kit
The GeneChip™ Hybridization, Wash, and Stain Kit is used with the GeneChip Brand Arrays in cartridge format, following the targetRead more
| Catalog Number | Quantity |
|---|---|
| 900720 | 1 kit |
Catalog number 900720
Price (USD)
864.00
Each
-
Quantity:
1 kit
Price (USD)
864.00
Each
The GeneChip™ Hybridization, Wash, and Stain Kit is used with the GeneChip Brand Arrays in cartridge format, following the target preparation steps described for 3' eukaryotic, prokaryotic, and exon assays.
For convenience, the Hybridization, Wash, and Stain Kit reagents are formulated into the fewest number of individual components possible, reducing preparation and handling steps. Most of the reagents are ready-to-use solutions.
The kit includes pre-formulated solutions to process 30 arrays on the GeneChip Fluidics Station 400 or 450.
Component Name Volume
Box 1 of 2
Hybridization Module
Pre-Hybridization Module 6 mL
2X Hybridization Mix 4.5 mL
DMSO 0.9 mL
Nuclease-free water 4 mL
Stain Module
- Stain Cocktail 1 18 mL
- Stain Cocktail 2 18 mL
Array Holding Buffer 30 mL
Nuclease-free water 4 mL
Box 2 of 2
Wash Buffer A* 3 bottles, 800 mL/bottle
Wash Buffer B* 1 bottle, 600 mL/bottle
*Wash Buffer A and Wash Buffer B are also available for purchase individually.
Related Links
Clariom™ D Assay, human
Clariom™ D Pico Assay, human
For convenience, the Hybridization, Wash, and Stain Kit reagents are formulated into the fewest number of individual components possible, reducing preparation and handling steps. Most of the reagents are ready-to-use solutions.
The kit includes pre-formulated solutions to process 30 arrays on the GeneChip Fluidics Station 400 or 450.
Component Name Volume
Box 1 of 2
Hybridization Module
Pre-Hybridization Module 6 mL
2X Hybridization Mix 4.5 mL
DMSO 0.9 mL
Nuclease-free water 4 mL
Stain Module
- Stain Cocktail 1 18 mL
- Stain Cocktail 2 18 mL
Array Holding Buffer 30 mL
Nuclease-free water 4 mL
Box 2 of 2
Wash Buffer A* 3 bottles, 800 mL/bottle
Wash Buffer B* 1 bottle, 600 mL/bottle
*Wash Buffer A and Wash Buffer B are also available for purchase individually.
Related Links
Clariom™ D Assay, human
Clariom™ D Pico Assay, human
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transcriptome Profiling
Product LineGeneChip™
Quantity1 kit
Reagent TypeHybridization, Wash, Stain
TypeHybridization, Wash, Stain Kit
Unit SizeEach
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Product Information
Frequently asked questions (FAQs)
No, we do not recommend using the GeneChip HWS kit for mapping arrays.
We recommend using the SNP 6 Core Reagent Kit (Cat. No. 901706) for the SNP array 6.0, which includes a hybridization, wash, and stain component. See this link: http://www.affymetrix.com/estore/browse/products.jsp?productId=prod460004&categoryId=35586&productName=Affymetrix%26%23174%3B-SNP-6-Core-Reagent-Kit#1_1
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Unfortunately, the staining components (SAPE and Ab) would not work after being frozen at -20 degrees C and therefore we do not recommend moving forward with staining.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
The Hybridization Controls are high-quality controls for monitoring array hybridization, washing, and staining for reproducible results.
20X Hybridization Controls are composed of a mixture of biotinylated and fragmented cRNA of bioB, bioC, and bioD from E. coli and cre from P1 bacteriophage in staggered concentrations. The premixed controls are ready to be added directly to the hybridization cocktail. Probes for detecting these controls are present on GeneChip miRNA 4.0 Array, Affymetrix miRNA 4.1 Array Strip, and Affymetrix miRNA 4.1 Array Plate.
The 20X Hybridization Controls are spiked into the hybridization cocktail, independent of RNA sample preparation, and are thus used to evaluate sample hybridization efficiency on eukaryotic gene expression arrays.
As the 20X Hybridization Controls are used to troubleshoot potential array and array processing issues, a failure to include the 20X Hybridization Controls in the hybridization cocktail will void the customer's ability to submit an array replacement request.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
The GeneChip HT hybridization, Wash Stain Kit (Cat. No. 901219) is part of an older kit that is discontinued and no longer available.
The kit consisted of:
- HT Pre-Hyb mix, HT 1.2X Hyb mix, HT Stain Cocktail 1& 3, HT Stain Cocktail 2, HT Wash Buffers A, HT Wash Buffer B, and HT Array Holding Buffer.
The GeneChip HWS kit (Cat. No. 900720) is a current product that can be ordered.
This kit consists of:
- Pre-Hyb module, 2X Hyb mix, DMSO, Nuclease Free Water, Stain Module (Stain Cocktail 1 and Stain Cocktail 2) Wash Buffers, Wash Buffer B, and Array Holding Buffer.
The HT kit was formulated for high throughput usage. Therefore, the formulations differ. The Wash buffers A and B are similar, but they have slight variations in terms of additives.
The Hyb mixes have different concentrations. The stain cocktails have different stain mixtures (Stain 1 & Stain 3 vs Stain 1).
Therefore, we do not recommend swapping any of these reagents.
Only the Array Holding Buffer is the same and could be swapped.
The exact components and concentrations of the reagents are proprietary information and thus cannot be disclosed.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.