FlashTag™ Biotin HSR RNA Labeling Kits
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Applied Biosystems™

FlashTag™ Biotin HSR RNA Labeling Kits

FlashTag™ Biotin HSR RNA Labeling Kits are high-sensitivity labeling reagents designed to prepare target from RNA samples for GeneChip™ miRNARead more
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Catalog NumberNo. of Reactions
90191130 Reactions
90191010 Reactions
Catalog number 901911
Price (EUR)
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No. of Reactions:
30 Reactions
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FlashTag™ Biotin HSR RNA Labeling Kits are high-sensitivity labeling reagents designed to prepare target from RNA samples for GeneChip™ miRNA Arrays.

Features and benefits
Easy-to-use: 45-minute assay—from RNA sample to labeled target—without amplification or purification steps
Low sample input: Requires as little as 130 ng total RNA
Variety of samples: Works with any intact, degraded, or FFPE RNA sample
Built-in controls: Contains RNA spike control oligos for GeneChip miRNA Arrays
Superior performance:
    - Reproducibility: Inter-and intra-lot signal correlation is typically R >0.95
    - Sensitivity: Detects 94% of miRNA transcripts at 1.0 attomole
    - Dynamic range: >3 logs
    - Specificity: 1 nucleotide discrimination

The combination of GeneChip miRNA Arrays and FlashTag Biotin HSR Reagents offers a complete solution for accurate and validated miRNA analysis.

Related Links
FlashTag™ Biotin HSR RNA Labeling Kits
GeneChip™ miRNA 4.1 24-Array Plate and Trays
GeneChip™ miRNA 4.1 96-Array Plate and Trays
GeneChip™ miRNA 3.1 24-Array Plate and Trays
GeneChip™ miRNA 3.1 96-Array Plate and Trays
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodBiotin-based
FormatKit
Final Product TypeRNA (Labeled)
Sample TypeRNA
Labeling TargetRNA (General)
Includes Label or DyeDirect Labeling
No. of Reactions30 Reactions
Product TypeRNA Labeling Kit
Quantity30 reactions
Unit SizeEach
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3225091Certificate of AnalysisApr 24, 2025901910
3182775Certificate of AnalysisMar 19, 2025901911
K08E23HSR1Certificate of AnalysisDec 16, 2024901910
3129662Certificate of AnalysisDec 13, 2024901911
3124985Certificate of AnalysisDec 09, 2024901910
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Safety Data Sheets

Frequently asked questions (FAQs)

The ERCC controls are not tiled onto GeneChip miRNA arrays because they would not work with the miRNA array/assay system. The FlashTag Biotin HSR RNA Labeling Kits used to label miRNAs is optimized for shortRNAs. Even though longer mRNAs like the ERCC controls will be labeled, the HWS (hyb/wash/stain) conditions are not optimal and consequently, the longer mRNAs typically do not get detected.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Due to certain miRNAs being conserved across species, there may be multiple copies of the same probe across the miRNA arrays. However, sample processing with the FlashTag Biotin HSR RNA Labeling Kits ensures that there is excess target compared to the probes on the array. Consequently, there is enough sample available to hybridize onto all the replicate probes.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

In order to perform RNA isolation, any of our kits for purification of total RNA or LMW RNA will be compatible with the FlashTag Biotin HSR RNA Labeling Kits.We also recommend that you elute or resuspend the RNA in nuclease-free water to ensure that the purification method retains low mole.

Reverse transcribed miRNA would not work because it would be the wrong sense (antisense, while our probes interrogate sense RNA). Amplified miRNA should work, but the miRNAs are very short. Therefore, adapters may need to be ligated to them or tailing may be needed to get enough runway to complete amplification. It is unknown if any extra sequence may interfere with the hybridization.

If you are using a FlashTag Biotin HSR RNA Labeling Kit for the GeneChip miRNA 3.1 Array Strip, we recommend staying within the range of 130-1000 ng input total RNA.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

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