GeneChip™ Human Transcriptome Array 2.0
GeneChip™ Human Transcriptome Array 2.0
Applied Biosystems™

GeneChip™ Human Transcriptome Array 2.0

Designed to empower next-generation expression profiling studies, GeneChip™ Human Transcriptome Array 2.0 provides the ability to go beyond gene-level expressionRead more
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Catalog NumberNumber of Arrays
90216210 arrays
9022332 arrays
Catalog number 902162
Price (USD)
4,250.00
Each
Add to cart
Number of Arrays:
10 arrays
Price (USD)
4,250.00
Each
Add to cart
Designed to empower next-generation expression profiling studies, GeneChip™ Human Transcriptome Array 2.0 provides the ability to go beyond gene-level expression profiling by providing the coverage and accuracy required to accurately detect all known transcript isoforms produced by a gene.

View the data sheet for details on HTA 2.0 content and coverage.

Comprehensive exploration of the transcriptome
Research has shown that the tens of thousands of human genes contain hundreds of thousands of exons, which produce hundreds of thousands of different transcript isoforms. These transcript isoforms are produced when the exons of a gene may be included within, or excluded from, the final, processed messenger RNA produced from that gene. Until now, measuring and analyzing these transcript isoforms has been nearly impossible due to technology limitations, sample input requirements, and lack of analysis capabilities/tools.

Comprehensive transcriptome analysis requires combining transcript diversity from multiple data sources
Most genes produce multiple transcript isoforms, and measuring changes in the relative abundance of each isoform provides new insights into disease and biology. HTA 2.0 has combined multiple data sources to ensure you are able to independently analyze the broadest collection of transcript isoforms available.

Data sources used to design and annotate the array
RefSeq                                    Vertebrate Genome Annotation (Vega) database
Ensembl                                   MGC Mammalian Gene Collection (v10)
UCSC Known Genes               www.noncode.org
UCSC lincRNA transcripts        lncRNA db
Broad Institute, Human Body Map lincRNAs, and TUCP (transcripts of uncertain coding potential) catalog

Better data than 2 full lanes of sequencing
See the HTA 2.0 Flyer in the Documents Section below for additional information. HTA 2.0 provides superior accuracy and precision coupled with the most comprehensive view of the transcriptome.

Bioinformatics built into the array design; no assembly required
HTA 2.0 maximizes the amount of unique and valuable information possible by minimizing the conserved sequence synthesized on the array. This high-resolution array design contains an unprecedented >6.0 million probes covering coding transcripts and non-coding transcripts. 70% of the probes on this array cover exons for coding transcripts, and the remaining 30% of probes on the array cover exon-exon splice junctions and non-coding transcripts. The unparalleled coverage of this array provides the deepest insight into all coding and non-coding transcripts available.

Simple, fast, and free analysis solution
For the first time ever, HTA 2.0 coupled with Expression Console™ Software and TAC Software offers researchers a complete solution from data to decision making in minutes. This complete analysis solution is provided to all researchers using our expression arrays at no additional cost. In addition, HTA 2.0 data analysis is supported by the same analysis solutions and service providers being used for other expression array data.

Related Links
GeneChip™ Hybridization, Wash, and Stain Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineGeneChip™
Quantity10 arrays
TypeHuman Transcriptome Array 2.0
ArrayTranscriptome Profiling
FormatArray Cartridge
Number of Arrays10 arrays
SpeciesHuman
Unit SizeEach

Frequently asked questions (FAQs)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How long does it take for the GeneChip Human Transcriptome Array 2.0 (Cat. No. 902162) to scan?

The GeneChip Human Transcriptome Assay 2.0 takes approximately 35 min to scan.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Why are multiple gene symbols associated with the same Transcript Cluster IDs in GeneChip Human Transcriptome Array 2.0?

Sometimes, there are transcript clusters in the GeneChip Human Transcriptome Array (HTA) 2.0 with multiple associated gene symbols, and it appears that the probes in the cluster span multiple adjacent/overlapping genes.

For example: The transcript TC04000948.hg.1 is associated with the gene symbols ABCA11P//ZNF721 in the GeneChip HTA 2.0 annotation file. This is one of those infrequent cases where multiple genes will co-exist in the same location. In this particular case, one of them is a zinc-finger protein, and the other one is a pseudogene. Pseudogenes are often defined as "not having any known function" or "not expressed". However, as the knowledge of biology progresses, the definition of "function" changes and it may be found that these do have function, just that it is not known. Pseudogenes occasionally are transcribed, and sometimes, they are just artifacts of ancient or recent duplication events.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the difference between GeneChip Human Transcriptome Array v1(HTAv1) and GeneChip Human Transcriptome Array 2.0 (HTA 2.0)?

HTAv1, also known as Glue Grant Human Transcriptome Array (GG-H), is a custom array made in collaboration with Stanford Genome Technology, Wing Wong's lab at Stanford, and the Inflammation and Host Response to Injury program (“Glue Grant”). The array was designed to interrogate gene expression, alternative splicing, detection of coding SNPs and non-coding transcription. Permissions from the group are required to use this array. HTA 2.0 is one of our catalog arrays. This array follows a similar design as the HTAv1 but focus on the whole transcript gene expression and alternative splicing of coding and non-coding transcripts. We provide assay and software for the processing and analysis of this array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How is this array design principle different from the GeneChip Human Genome U133 type of arrays?

The GeneChip Exon Arrays represent a new array design philosophy for the most comprehensive and informative coverage with the exon as the basic unit of expression analysis. Such an inclusive design enables discovery of new and novel splicing events not previously observed experimentally. Some of the key differences are summarized below using the human arrays as an example; refer to the “GeneChip Exon Array Design” Technical Note and the “Design and Performance of the GeneChip Human Genome U133 Plus 2.0 and Human Genome U133A 2.0 Array” Technical Note for more details.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

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