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StemPro™ hESC SFM
Developed for human embryonic and human induced pluripotent stem cell cultureStemPro™ hESC SFM is a fully defined, serum- and feeder-freeRead more
| Catalog Number | Quantity |
|---|---|
| A1000701 | 1 Kit |
Catalog number A1000701
Price (USD)
522.00
Each
-
Quantity:
1 Kit
Price (USD)
522.00
Each
Developed for human embryonic and human induced pluripotent stem cell culture
StemPro™ hESC SFM is a fully defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of human embryonic (hESCs) and human induced pluripotent stem cells (iPSC). StemPro™ hESC SFM has been extensively tested and is proven to maintain pluripotency in a growing list of hESC lines including BG01, BG02, BG03, H1, H9, and BG01V. In addition, StemPro™ hESC SFM has been used to scale up production of hESCs to over 1 x 1010 cells. StemPro™ hESC SFM has been shown to support hESC and iPSC growth for >50 passages without any signs of karyotypic abnormalities, and to maintain the ability of hESCs to differentiate into all three germ line lineages.
StemPro™ hESC SFM Components:
StemPro™ hESC Supplement
DMEM⁄F-12 with GlutaMAX™ medium
Bovine serum albumin 25% (BSA)
Additional components required for complete medium (need to be purchased separately):
FGF BASIC (full length) REC HU (Cat. no. PHG0261)
2-Mercaptoethanol (Cat. no. 21985-023)
Applications:
Formulated for the growth and expansion of human embryonic and human induced pluripotent stem cells.
StemPro™ hESC SFM is a fully defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of human embryonic (hESCs) and human induced pluripotent stem cells (iPSC). StemPro™ hESC SFM has been extensively tested and is proven to maintain pluripotency in a growing list of hESC lines including BG01, BG02, BG03, H1, H9, and BG01V. In addition, StemPro™ hESC SFM has been used to scale up production of hESCs to over 1 x 1010 cells. StemPro™ hESC SFM has been shown to support hESC and iPSC growth for >50 passages without any signs of karyotypic abnormalities, and to maintain the ability of hESCs to differentiate into all three germ line lineages.
StemPro™ hESC SFM Components:
StemPro™ hESC Supplement
DMEM⁄F-12 with GlutaMAX™ medium
Bovine serum albumin 25% (BSA)
Additional components required for complete medium (need to be purchased separately):
FGF BASIC (full length) REC HU (Cat. no. PHG0261)
2-Mercaptoethanol (Cat. no. 21985-023)
Applications:
Formulated for the growth and expansion of human embryonic and human induced pluripotent stem cells.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEmbryonic Stem Cells (ESCs)
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product LineStemPro™
Product TypeStem Cell Media
Quantity1 Kit
Shipping ConditionSupplement - dry ice, Basal - ambient, BSA - ambient
SpeciesHuman
ClassificationSerum-free
Culture TypeFeeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic)
FormLiquid
Serum LevelSerum-free
With AdditivesGlutaMAX, Phenol Red
Unit SizeEach
Contents & Storage
• StemPro™ hESC Supplement, store at -5 to -20°C and protect from light
• DMEM/F-12 with GlutaMAX medium, store at 2 to 8°C and protect from light
• Bovine serum albumin (BSA) 25%, store at 2 to 8°C and protect from light
• DMEM/F-12 with GlutaMAX medium, store at 2 to 8°C and protect from light
• Bovine serum albumin (BSA) 25%, store at 2 to 8°C and protect from light
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Figures
Robust single cell passaging of hESCs.
Human iPSC staining using Mouse Anti Beta-Tubulin Monoclonal Antibody.
Human iPSC staining.
Human iPSC staining using mouse anti beta-tubulin monoclonal antibody.
Human iPSC staining using mouse anti beta-tubulin monoclonal antibody.
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, we don't recommend StemPro hESC SFM for mouse cells because they showed poor growth and some differentiation. We recommend KnockOut DMEM and KnockOut SR for mouse ESC cultures (catalog numbers 10829-018 and 10828-028 respectively).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, derivations using StemPro hESC SFM have been carried out using either a basement membrane extract (like Geltrex growth factor) or feeder cells.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
StemPro hESC SFM has been tested with the following hESC: H1, H9, BG01, BG02, BG03, HUES6, HUES9, Cyth203, CyT49, BG01v, HES-2, HES-3, KhES-1, and TE06. The medium has also been used with the Rhesus monkey line R336.4. Some cells may require a short adaptation time but most do not.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, we recommend using Geltrex hESC-qualified Reduced Growth Factor basement membrane (catalog number A10480-02), CELLstart (catalog number A10142-01) or similar matrix.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.