ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits)
ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits)
Invitrogen™

ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits)

ArC™ Amine Reactive Compensation Bead Kit is a bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cellRead more
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Catalog NumberNo. of Tests
A10346100
A1062825
Catalog number A10346
Price (USD)
448.00
Each
-
Add to cart
No. of Tests:
100
Price (USD)
448.00
Each
Add to cart
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ArC™ Amine Reactive Compensation Bead Kit is a bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cell stain kits.

• Ready-to-use control
• Eliminates the hassle of heat-treating cells as a control, saving precious sample
• Enables accurate and consistent results

View a selection guide for all fixable viability dyes for flow cytometry.

Ready-to-use control
Unlike other methods that require sample preparation to make compensation controls using cellular material, the ArC Amine Reactive Compensation Bead Kit is ready to use out of the dropper bottle. The kit includes two types of specially modified polystyrene microspheres that have been optimized to react with the amine-reactive dye in the LIVE/DEAD Fixable dead cell stain kits. The ArC reactive beads (Component A) bind any of the amine-reactive dyes and provide a positive signal; the ArC negative beads (Component B) have no reactivity and provide a negative compensation control. This ready-to-use product preserves the original sample material for experiments; precious sample is not used for the creation of compensation controls.

Accurate and consistent results
Unlike biological controls, which can have variable expression levels depending upon disease or development state, the Arc beads provide a bright, consistent signal (see figure below). Consistency in the staining pattern of compensation controls is important for obtaining the right compensation value. If the signal varies with the control material used, the compensation value can be affected, leading to erroneous results.

How it works
One drop of beads is added to a test tube containing LIVE/DEAD Fixable dead cell stain. After incubation with amine-reactive dye, the beads are washed in staining buffer, a drop of negative control beads is added (if required), and the beads are resuspended and analyzed by flow cytometry. The two components provide a distinct positive bead that is at least 50-fold brighter than the negative beads, which can be used to set compensation.

Other compensation products
Besides the ArC Amine Reactive Compensation Beads, AbC™ Anti-Mouse Bead Kit is also available for capture of mouse anti-human antibodies, as well as the AbC™ Anti-Rat/Hamster Bead Kit for capture of rat anti-mouse or hamster anti-mouse antibodies.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Diameter (Metric)6 μm
Excitation Wavelength Rangeany lasers (UV to 633/635)
For Use With (Equipment)Flow Cytometer
FormatTube
No. of Tests100
Quantity1 Kit
Shipping ConditionRoom Temperature
Stain ConfigurationSurface
Product LineCompenFlow™, Molecular Probes™
Product TypeCompensation Beads
Unit SizeEach
Contents & Storage
Contains 1 vial ArC™ amine reactive beads and 1 vial of negative beads.
  • Store at 2°C to 8°C
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    Frequently asked questions (FAQs)

    What is the stability for the ArC Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD Fixable dead cell stain kits)?

    When stored as directed (2-8 degrees C), this kit is stable for at least 1 year. Please do not freeze the kit. Please visit the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/arc_amine_reactive_comp_bead_kit_man.pdf) for further technical details.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    My ArC Amine Reactive Compensation beads are excessively clumped and cannot be resuspended by vortexing or sonication. What may have caused this?

    This can occur if the solution of beads was ever frozen. The formation of ice excludes the beads and compresses the beads together. Once frozen, the beads cannot be resuspended into solution and are no longer usable.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    For the ArC Amine Reactive Compensation Bead Kit, why must I use freshly prepared amine-reactive dye?

    This is because succinimidyl ester (NHS ester) groups are very labile to water; they dissociate readily from the dye structure if held in solvents that are contaminated with water, as is possible from condensation or absorbing water from the atmosphere.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Besides the Live/Dead Fixable Dead Cell stain, may I conjugate other amine-reactive dyes to the beads in the ArC Amine Reactive Compensation Bead Kit?

    Yes. The succinimidyl ester (NHS ester) and isothiocyanate (e.g., FITC, TRITC) dye derivatives may be attached to the ArC beads, but you would have to optimize the amount of reactive dye per ArC beads.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    I am performing flow cytometry. My compensation matrix has values over 100. That's not possible is it?

    It is possible. But if you have a lot of values over 100, you probably need to look at the voltages that you are using for your data collection.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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