Alexa Fluor™ 568 Hydrazide

Invitrogen™

Alexa Fluor™ 568 Hydrazide

Alexa Fluor™ 568 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketonesRead more

Catalog Number	Quantity
A10437	1 mg

Catalog number A10437

Price (USD)

444.00

Each

Add to cart

Quantity:

1 mg

Price (USD)

444.00

Each

Add to cart

Alexa Fluor™ 568 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 568 is a bright, red fluorescent dye. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 568 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor™ 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection (learn more).

Detailed information about this AlexaFluor™ hydrazide:

• Fluorophore label : Alexa Fluor™ 568 dye
• Reactive group: hydrazide
• Reactivity: Aldehydes or keytones in polysaccharides or glycoproteins
• Ex/Em of the conjugate: 576/599 nm
• Extinction coefficient: 86,000 cm-1M-1
• Spectrally similar dyes: Rhodamine Red
• Molecular weight: 730.74

Cell Tracking and Tracing Applications
Alexa Fluor™ hydrazides and hydroxlamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent. Learn more about cell tracking and tracing.

Glycoprotein and Polysaccharide Labeling Applications
The Alexa Fluor™ hydrazides and hydroxlamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.

Hydrazide vs Hydroxylamine
Hydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH₄) to further increase the stability of the linkage.

Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook.

We’ll Make a Custom Conjugate for You
If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

Related Products
DMSO (dimethylsulfoxide) (D12345)
Antibody Conjugate Purification Kit for 0.5-1 mg (A33086)
Antibody Conjugate Purification Kit for 20-50 μg (A33087)
Antibody Conjugate Purification kit for 50-100 μg (A33088)

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical ReactivityCarboxylic Acid, Ketone, Aldehyde

Emission599 nm

Excitation576 nm

Label or DyeAlexa Fluor™ 568

Product TypeHydrazide

Quantity1 mg

Reactive MoietyAmine, Hydrazide

Shipping ConditionRoom Temperature

Label TypeAlexa Fluor

Product LineAlexa Fluor™

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

What dye can I use that is non-reactive and can show an injection site?

The non-reactive Alexa Fluor and Alexa Fluor hydrazide derivatives may be used for injection site visualization. Other options include the fluorescent polystyrene microspheres, FluoSpheres, and dye-conjugated dextrans. The hydrazide derivatives and 'fixable' dextrans are retained by cross-linking using an aldehyde-based fixative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I injected a fluorescent tracer, but cannot detect it after tissue is fixed and sectioned. What am I doing wrong?

Confirm that the tracer you are using crosslinks to proteins or has a primary amine for fixation-either a hydrazide, lysine fixable dextran, or a protein conjugate.
Use aldehyde-based fixatives to cross link the amines on the tracer.
Inject a larger amount or higher concentration of the tracer. Tracers are generally injected at 1-20% concentrations (10 mg/mL or higher).
Confirm that you are using the correct fluorescent filter for detection. You can perform a spot test by pipetting a small amount of the undiluted stock solution of the tracer onto a slide, then view under the filter you are using on your microscope. This will confirm if the tracer fluorescence can be detected and the fluorescent microscope filter is working properly.
Review tissue fixation and handling procedures to confirm if any reagents or processing procedures could be affecting the tracer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have labeled my neurons with an Alexa Fluor conjugated biocytin to look at transport but I wanted to examine only retrograde transport and biocytin appears to be moving retrograde and anterograde. What should I do?

Observing both types of transport is typical for biocytin. The conjugated cholera toxin subunit B products have been observed to travel only retrogradely.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have a neuronal tracer similar to Lucifer Yellow but in another fluorescent color?

Lucifer Yellow CH is a hydrazide, so any of our Alexa Fluor or fluorescent hydrazides could potentially be used. A listing of them can be found here. (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing/hydrazides-biocytins.html#prd)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I know which tracer to choose for my experiment?

Factors to consider are size of tracer, method of delivery (injection, direct application to tissue, etc.), and if the tracer needs to be fixable. Here are some links to details about the various classes of neuronal tracers we offer and how to choose between them:

Neuronal Tracing (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)
Choosing a Tracer (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/choosing-a-tracer.html)
Imaging Analysis (http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/bioprobes-50-journal.pdf)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Alexa Fluor™ 568 Hydrazide FAQs

Documents & Downloads

Certificates

Search by lot number or partial lot number

Request a Certificate

Alexa Fluor™ 568 Hydrazide

Frequently asked questions (FAQs)

What dye can I use that is non-reactive and can show an injection site?

I injected a fluorescent tracer, but cannot detect it after tissue is fixed and sectioned. What am I doing wrong?

I have labeled my neurons with an Alexa Fluor conjugated biocytin to look at transport but I wanted to examine only retrograde transport and biocytin appears to be moving retrograde and anterograde. What should I do?

Do you have a neuronal tracer similar to Lucifer Yellow but in another fluorescent color?

How do I know which tracer to choose for my experiment?

Documents & Downloads

Certificates

Safety Data Sheets