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Invitrogen™
Jump-In™ Fast Gateway™ System
Catalog number: A10893
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Invitrogen™
Jump-In™ Fast Gateway™ System
Catalog number: A10893
Have Questions? Catalog Number
A10893
Unit Size
20 reactions
Price (USD)
6,900.00
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| A10893 | 20 reactions | 6,900.00 | - |
Product Overview
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The Jump-In™ Fast Gateway™ System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway™ System consists of:
• The Jump-In™ Fast Gateway™ Core Kit containing the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
• The MultiSite Gateway™ Pro Plus kit for cloning of up to 4 gene fragments into the promoter-less pJTI™ Fast DEST expression vector.
The Jump-In™ Fast Gateway™ System consists of:
• The Jump-In™ Fast Gateway™ Core Kit containing the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
• The MultiSite Gateway™ Pro Plus kit for cloning of up to 4 gene fragments into the promoter-less pJTI™ Fast DEST expression vector.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning Method
Gateway™
Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Cell Type
Mammalian
Promoter
None (Promoterless)
Vector
Jump-In Vectors, pJTI
Expression System
Mammalian
For Use With (Application)
Protein Expression
High-throughput Compatibility
High-throughput Compatible
Key Functions
Stable Cell Line Development, Targeted Integration, Clone your own Promoter
No. of Reactions
20
Product Line
Jump-In™
Product Type
Cloning Kit
Protein Tag
Untagged
Quantity
20 Reactions
Selection Agent (Eukaryotic)
Hygromycin
Selection Marker Eukaryotic
HygroR
Contents & Storage
Jump-In™ Fast Gateway™ System is composed of the Jump-In™ Fast Gateway™ Core Kit and the MultiSite Gateway™ Pro Plus Kit (includes One Shot™ Mach1™ T1R Chemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes).
This kit has components that can also be sold separately.
Store cells at -80°C.
Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage.
Store all other components, including vectors, at -20°C.
This kit has components that can also be sold separately.
Store cells at -80°C.
Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage.
Store all other components, including vectors, at -20°C.
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