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StemPro™ Accutase™ Cell Dissociation Reagent
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StemPro™ Accutase™ Cell Dissociation Reagent
Gibco™

StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes.
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Catalog NumberQuantity
A1110501100 mL
Catalog number A1110501
Price (USD)
75.00
Each
In stock
Add to cart
Quantity:
100 mL
Price (USD)
75.00
Each
Add to cart
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Cell lines tested for Accutase® application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells and primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, and established cell lines such as adherent CHO and BHK cells and 293 cells. A complete list of published references is available on our website. Accutase® is a trademark of ICT, Inc.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells
Culture TypeAdherent Cell Culture
Inorganic SaltsSodium Phosphate
Product LineGibco™, StemPro™
Purity or Quality GradeResearch Grade
Quantity100 mL
Serum LevelSerum-Free
Shelf Life24 Months
Shipping ConditionDry Ice
SourceSerum-Free
ClassificationSerum-free
FormFrozen
Product TypeCell Culture Dissociation Reagent
pH6 to 8
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C) and protect from light.

Frequently asked questions (FAQs)

Do I need to worry about over-dissociating my cells with StemPro Accutase Cell Dissociation Reagent?

No. However, although StemPro Accutase is gentle on cells, the optimal time for dissociation should be empirically determined for your specific cell type and application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to dilute StemPro Accutase Cell Dissociation Reagent before using it?

No. StemPro Accutase is supplied as a convenient, ready to use reagent. No dilution is required prior to use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

With StemPro Accutase Cell Dissociation Reagent, do I need to stop the dissociation reaction with serum?

No. StemPro Accutase is gentle enough that only dilution of the reagent with DPBS or media is required to stop the dissociation activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store StemPro Accutase Cell Dissociation Reagent at 2 to 8 degrees C?

We recommend storing StemPro Accutase Cell Dissociation Reagent at -5 to -20 degrees C, protected from light. We do not recommend subjecting it to more than 3-4 freeze-thaws. When stored at 2 to 8 degrees C, the reagent is stable for up to 2 years.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I accidentally thawed the StemPro Accutase Cell Dissociation Reagent at 37 degrees C. Can I still use it?

Yes. If StemPro Accutase is exposed to 37 degrees C just until complete thawing is achieved, it still can be used. However, this could result in decreased enzyme activity and it would take more time to get full dissociation. If you observe this, consider starting with a new bottle. Please note that if a bottle of StemPro Accutase is kept at 37 degrees C for more than one hour, it will lose its activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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