Our Alexa Fluor 350 conjugate of NeutrAvidin biotin-binding protein – a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point – can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding. This bright, blue-fluorescent reagent (absorption/emission maxima ∼346/442 nm) is ideal for detecting biotinylated targets in fluorescence microscopy and flow cytometry applications.