Phalloidin Labeling Probes
Phalloidin Labeling Probes
Invitrogen™

Phalloidin Labeling Probes

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.
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货号颜色激发波长范围染料类型
A12381Red581⁄609Alexa Fluor™ 594
A22281Blue346⁄442Alexa Fluor™ 350
A30104Violet405/450Alexa Fluor™ Plus 405
A12379Green激发波长495 nm 发射波长518 nmAlexa Fluor™ 488
O7466Green496⁄520Oregon Green™ 488
F432Green496⁄516FITC(荧光素)
A22282Yellow531⁄554Alexa Fluor™ 532
R415Red-orange540⁄565TRITC(四甲基异硫氰酸罗达明)
A22283Orange556⁄570Alexa Fluor™ 546
A34055Orange555⁄565Alexa Fluor™ 555
A30106Orange555/565 nmAlexa Fluor Plus 555
B3475Red558⁄569BODIPY™
A12380Orange-red578⁄600Alexa Fluor™ 568
T7471Red591⁄608Texas Red™
A22284Far-red632⁄647Alexa Fluor™ 633
A34054Far-red633⁄647Alexa Fluor™ 635
A22287Far-red650⁄668Alexa Fluor™ 647
A30107Far-red650/668 nmAlexa Fluor Plus 647
A22285Near-infrared663⁄690Alexa Fluor™ 660
A22286Near-infrared679⁄702Alexa Fluor™ 680
A30105Near-infrared758/784Alexa Fluor™ Plus 750
货号 A12381
价格(CNY)
7,115.00
飞享价
Ends: 31-Dec-2025
9,644.00
共减 2,529.00 (26%)
Each
添加至购物车
颜色:
Red
激发波长范围:
581⁄609
染料类型:
Alexa Fluor™ 594
物流信息
常规产品: 发货后, 北上广深及省会城市通常为 1-2天,二线城市通常 2-3天,三线以及偏远地区通常 4天,周末以及节假日顺延。
空运受限产品: 发货后, 北上广深及省会城市通常为 2-4天,二线城市 通常3-5天,三线以及偏远地区通常 6-10天,周末以及节假日顺延。
现货中心: 此类产品将由您身边的现货中心极速配送。
价格(CNY)
7,115.00
飞享价
Ends: 31-Dec-2025
9,644.00
共减 2,529.00 (26%)
Each
添加至购物车
Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

  • High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
  • Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
  • Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
  • Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
  • Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
  • Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
  • Ease of use—staining is straightforward and quick
  • Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
  • Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton
仅供科研使用。不可用于诊断程序。
规格
颜色Red
染料类型Alexa Fluor™ 594
激发波长范围581⁄609
适用于(设备)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with Texas Red filter set
产品线Alexa Fluor™
数量300 Units
运输条件室温
标签类型Alexa Fluor 染料
产品类型鬼笔环肽
亚细胞定位肌动蛋白、细胞骨架, Cytoskeleton
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

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证书

批号Certificate TypeDateCatalog Number(s)
3188272Certificate of Analysis2025年6月29日A12381
3163213Certificate of Analysis2025年6月18日A22281
3203004Certificate of Analysis2025年6月18日A30107
3158259Certificate of Analysis2025年6月18日A22284
3148327Certificate of Analysis2025年5月28日A34055
显示 5 条结果, 在上面搜索特定证书

安全数据表

常见问题解答 (FAQ)

当细胞和组织经由二甲苯或丙酮之类的溶剂处理时(例如组织切片脱石蜡期间),它通过阻止鬼笔环肽结合的方式来影响F- actin。冰冻切片通常不使用有机溶剂洗涤,可以用来代替石蜡切片,也可以使用抗肌动蛋白抗体。

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (2496)

引用和文献
Abstract
Identification of the C-terminal part of Bordetella dermonecrotic toxin as a transglutaminase for rho GTPases.
Authors:Schmidt G,Goehring UM,Schirmer J,Lerm M,Aktories K
Journal:The Journal of biological chemistry
PubMed ID:10542213
DOCK2 is a Rac activator that regulates motility and polarity during neutrophil chemotaxis.
Authors:Kunisaki Y,Nishikimi A,Tanaka Y,Takii R,Noda M,Inayoshi A,Watanabe K,Sanematsu F,Sasazuki T,Sasaki T,Fukui Y
Journal:The Journal of cell biology
PubMed ID:16943182
Neutrophils are highly motile leukocytes, and they play important roles in the innate immune response to invading pathogens. Neutrophil chemotaxis requires Rac activation, yet the Rac activators functioning downstream of chemoattractant receptors remain to be determined. We show that DOCK2, which is a mammalian homologue of Caenorhabditis elegans CED-5 and ... More
A natural ErbB4 isoform that does not activate phosphoinositide 3-kinase mediates proliferation but not survival or chemotaxis.
Authors:Kainulainen V,Sundvall M,Määttä JA,Santiestevan E,Klagsbrun M,Elenius K
Journal:The Journal of biological chemistry
PubMed ID:10722704
The p42/p44 MAP kinase pathway prevents apoptosis induced by anchorage and serum removal.
Authors:Le Gall M,Chambard JC,Breittmayer JP,Grall D,Pouysségur J,Van Obberghen-Schilling E
Journal:Molecular biology of the cell
PubMed ID:10712523
Anchorage removal like growth factor removal induces apoptosis. In the present study we have characterized signaling pathways that can prevent this cell death using a highly growth factor– and anchorage-dependent line of lung fibroblasts (CCL39). After anchorage removal from exponentially growing cells, annexin V-FITC labeling can be detected after 8 ... More
Authors:
Journal:
PubMed ID:10896672
2496 total citations

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