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货号 | 颜色 | 激发波长范围 | 染料类型 |
---|---|---|---|
A12381 | Red | 581⁄609 | Alexa Fluor™ 594 |
A22281 | Blue | 346⁄442 | Alexa Fluor™ 350 |
A30104 | Violet | 405/450 | Alexa Fluor™ Plus 405 |
A12379 | Green | 激发波长495 nm 发射波长518 nm | Alexa Fluor™ 488 |
O7466 | Green | 496⁄520 | Oregon Green™ 488 |
F432 | Green | 496⁄516 | FITC(荧光素) |
A22282 | Yellow | 531⁄554 | Alexa Fluor™ 532 |
R415 | Red-orange | 540⁄565 | TRITC(四甲基异硫氰酸罗达明) |
A22283 | Orange | 556⁄570 | Alexa Fluor™ 546 |
A34055 | Orange | 555⁄565 | Alexa Fluor™ 555 |
A30106 | Orange | 555/565 nm | Alexa Fluor Plus 555 |
B3475 | Red | 558⁄569 | BODIPY™ |
A12380 | Orange-red | 578⁄600 | Alexa Fluor™ 568 |
T7471 | Red | 591⁄608 | Texas Red™ |
A22284 | Far-red | 632⁄647 | Alexa Fluor™ 633 |
A34054 | Far-red | 633⁄647 | Alexa Fluor™ 635 |
A22287 | Far-red | 650⁄668 | Alexa Fluor™ 647 |
A30107 | Far-red | 650/668 nm | Alexa Fluor Plus 647 |
A22285 | Near-infrared | 663⁄690 | Alexa Fluor™ 660 |
A22286 | Near-infrared | 679⁄702 | Alexa Fluor™ 680 |
A30105 | Near-infrared | 758/784 | Alexa Fluor™ Plus 750 |
A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.
Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.
Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining
Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.
Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.
Features of phalloidin probes
当细胞和组织经由二甲苯或丙酮之类的溶剂处理时(例如组织切片脱石蜡期间),它通过阻止鬼笔环肽结合的方式来影响F- actin。冰冻切片通常不使用有机溶剂洗涤,可以用来代替石蜡切片,也可以使用抗肌动蛋白抗体。
We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.
For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.
Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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