Human Skeletal Myoblasts (HSkM)
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Gibco™

Human Skeletal Myoblasts (HSkM)

Human Skeletal Myoblasts
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Catalog NumberQuantity
A125551 vial x 106 cells
A114401 vial x 5x106 cells
Catalog number A12555
Price (USD)
428.00
Each
Add to cart
Quantity:
1 vial x 106 cells
Price (USD)
428.00
Each
Add to cart
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Primary Normal Human Skeletal Myoblasts (HSkM) are designed to undergo highly efficient differentiation directly following plating of cryopreserved cells. Each vial contains cells that have been cryopreserved in a medium containing 10% DMSO and will differentiate within 48 hours after plating. These cells do not require expansion prior to differentiation. Cell viability is >70% post thaw with a ≥50% myogenic index after 48 hours. Intended for use by researchers investigating the molecular and biochemical bases of various normal and disease processes. They are compatible with Bacmam delivery technologies for various downstream applications. Large size contains 4.5 x 10^6 ≤ N ≤ 7.5 x 10^6 cells per vial and small size contains 1.1 x 10^6 ≤ N ≤ 1.9 x 10^6 cells per vial.

Primary Normal Human Skeletal Myoblasts (HSkM) are designed to undergo highly efficient differentiation following plating of cryopreserved cells.

The cells are:

  • Performance tested—guaranteed to differentiate ≥ 50% following 48 hours of incubation
  • Guaranteed to be ≥ 70% viable
  • Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contain sufficient number of cells to fully seed a single multi-well dish (ranging in format from 6-well to 384-well).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
AgeAdult
Cell TypeSkeletal Muscle Cells
Culture TypeAdherent Cell Culture
FormCryopreserved
GenderMixed Gender
SpeciesHuman
Donor AttributesNormal
Donor SourceSingle Donor
FormatVial
No. of Cells1 x 10^6
Product LineGibco™
Product TypeHuman Skeletal Myoblast
Quantity1 vial x 106 cells
Tested ForMycoplasma, Bacteria, Yeast or Other Fungi, Hepatitis B, Hepatitis C, HIV-1 Virus
Unit SizeEach
Contents & Storage
Store in liquid nitrogen.
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Frequently asked questions (FAQs)

Have you performed any studies using the human skeletal myoblasts to replicate physiological conditions?

Please see the images below showing Gibco Human Skeletal Myoblasts responding to physiological levels of TGF-beta1 and IGF-1. TGF-beta1 addition inhibits differentiation, whereas the addition of IGF-1 (Cat. No. PHG0075) blunts this effect. The anti-troponin staining method was used.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

Are primary cells isolated from tissue using enzymatic digestion?

Yes, our primary cells are isolated from tissue using enzymatic digestion.

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