Lipofectamine™ LTX Reagent with PLUS™ Reagent
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Lipofectamine™ LTX Reagent with PLUS™ Reagent
Invitrogen™

Lipofectamine™ LTX Reagent with PLUS™ Reagent

Lipofectamine™ LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells,Read more
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Catalog NumberQuantity
A126210.1 mL
153380300.3 mL
153381001 mL
Catalog number A12621
Price (USD)
110.65
Online Exclusive
116.00
Save 5.35 (5%)
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Quantity:
0.1 mL
Price (USD)
110.65
Online Exclusive
116.00
Save 5.35 (5%)
Each
Add to cart
Lipofectamine™ LTX Reagent is a plasmid transfection reagent that offers the right balance of potency and gentleness for your cells, resulting in the highest possible transfection efficiencies and viabilities. Lipofectamine™ LTX Reagent is an advanced solution for gene expression in both common and challenging cell types. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine™ LTX Reagent for transfection of primary, challenging, and sensitive cell lines. Lipofectamine™ LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cell lines.

Lipofectamine™ LTX Reagent offers:

• Superior delivery—transfect a broad range of cell types, including primary, challenging, and sensitive cells, with high efficiency
• Superior performance—combine high transfection efficiency with maximal (>90%) cell viability
• Optimized protocols—rapid, simple protocols provided for most cell lines
• A highly effective reagent to generate stable cell lines

Transfect primary cells efficiently
Primary cells are often difficult to transfect. Lipofectamine™ LTX Reagent, when combined with PLUS™ Reagent, provides the highest level of transfection in primary cells of any lipid- or polymer-based reagent. Many primary cell types have been successfully transfected with Lipofectamine™ LTX Reagent, including primary neural progenitor cells.

Superior delivery into challenging cells
When it comes to achieving high levels of protein expression in RAW267.3 macrophages, Lipofectamine™ LTX Reagent outperforms competing transfection reagents (see figure). Achieving high-level transgene expression in cells that are traditionally refractory to transfection allows you to obtain results that were previously unattainable.

Broad compatibility with cell lines
In addition to being excellent for transfecting primary and challenging cells, Lipofectamine™ LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents. Use our Transfection Selection Tool to help you select the best transfection conditions for the cells with which you work.

Stable transfection
Lipofectamine™ LTX strikes the right balance between potency and gentleness, giving you the highest possible percentage of successfully transfected cells. This makes Lipofectamine™ LTX Reagent the quickest and easiest reagent for establishing stable transfectants from challenging or refractory cell types.

Simple, rapid, serum-compatible protocol
Lipofectamine™ LTX Reagent offers a streamlined protocol—no need to remove transfection complexes or change/add medium following transfection. A simple optimization protocol helps you get the best results quickly, so you'll spend less hands-on time, and get faster results.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-Free
For Use With (Application)Transfection
High-throughput CompatibilityHigh-throughput Compatible
Product LineLipofectamine™
Product TypeLTX Reagent
Quantity0.1 mL
RNAi TypeShRNA, miR
Serum CompatibleYes
Shipping ConditionWet Ice
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Each unit contains one vial of Lipofectamine™ LTX Reagent (0.1 mL) and one vial of Plus Reagent (40 μl). Store at 2–8°C.

Frequently asked questions (FAQs)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

Is it necessary to use serum-free medium during lipid transfection?

It is not necessary to use serum-free medium during lipid transfection. However, it is critical to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum-containing medium. For optimal results with Lipofectin Transfection Reagent, we recommend performing transfection in medium without serum.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

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